【摘要】 目的为了提高着床前遗传学筛查(PGT)周期患者体外授精-胚胎移植(IVF-ET)成功率和胚胎利用率,通过单核苷酸多态性-微阵列比较基因组杂交技术(array-SNP)分析PGT患者的2PN、0PN和1PN来源的胚胎染色体的整倍性,从而优化移植策略。方法回顾性分析2013—2018年在我中心行PGT助孕,其2PN、1PN和0PN来源的胚胎均有囊胚形成的患者,比较不同发育速度的2PN、0PN和1PN来源的活检囊胚的染色体整倍性。结果比较第5天不同受精来源的活检囊胚染色体整倍体率差异无统计学意义(P>0.05);第6天囊胚中,0PN来源的活检囊胚染色体整倍体率要低于2PN和1PN来源的活检囊胚整倍体率,差异有统计学意义(P<0.05);第5天和第6天的囊胚相比,不同受精来源的囊胚染色体整倍体率差异无统计学意义(P>0.05)。结论对于PGT周期患者,0PN以及1PN来源的胚胎培养后形成囊胚,通过芯片技术检测其染色体正常的囊胚仍占一定比例,可作为移植胚胎。不同发育速度的囊胚,其染色体的整倍体率差异并无统计学意义。更多还原

【Abstract】 Objective The aim to analyze the chromosome ploidy of 2 PN,0 PN and 1 PN-derived embryos in patients with preimplantation genetic testing(PGT) by array-SNP,in order to optimize the transplantation strategy,thereby improving the pregnancy rate and embryo utilization rate of IVF-ET. Methods In this retrospective study,we compared the chromosome ploidy of biopsy-derived blastocysts from 2 PN,0 PN and 1 PN with different development rates in the patients who had blastocyst formation in 2 PN,1 PN and 0 PN-derived embryos in our center from 2013 to 2018.Results There was no significant difference in chromosome ploidy rate between the blastocysts from different fertilization sources on day 5(P> 0.05). On day 6,the chromosome ploidy rate of 0 PN-derived blastocysts was significantly lower than that of 2 PN and 1 PN-derived blastocysts from biopsy(P <0.05). There was no significant difference in the chromosome ploidy rate of blastocysts from different sources between day 5 and day 6(P>0.05). Conclusion For the patients with PGT cycle,a certain proportion of blastocysts,formed from 0 PN and 1 PN-derived embryos,have normal chromosomes as determined by the chip technology,which could be used for embryo transplantation.更多还原