【摘要】 目的研究miR-214靶向Ras相关区域家族5(RASSF5)基因对口腔癌细胞CAL27生长活力的调节作用。方法收集邢台市人民医院手术切除的口腔癌组织和癌旁组织,检测miR-214、RASSF5的表达水平;培养口腔癌细胞CAL27,分为转染阴性对照(NC)模拟物的NC模拟物组、转染miR-214模拟物的miR-214模拟物组,检测细胞生长活力、细胞周期分布及细胞周期基因的表达水平。结果口腔癌组织中miR-214的表达水平明显高于癌旁组织,RASSF5的mRNA表达水平明显低于癌旁组织(P <0.05),且口腔癌组织中miR-214的表达水平与RASSF5的mRNA表达水平呈负相关(P <0.05); miR-214模拟物组口腔癌细胞CAL27细胞的生长活力值及S期、G2/M期比率均明显高于NC模拟物组,G0/G1期比率明显低于NC模拟物组且细胞中RASSF5、p53、p21的mRNA表达水平明显低于NC模拟物组,Cyclin D1、Cyclin E的mRNA表达水平明显高于NC模拟物组(P <0.05)。结论 miR-214通过靶向抑制RASSF5基因的表达能够增强口腔癌细胞CAL27的生长活力。更多还原

【Abstract】 Objectvie To investigate the regulatory effect of miR-214 on the growth viability of oral cancer cell CAL27 by targeting Ras association domain family5(RASSF5) gene.Methods Oral cancer tissues and adjacent tissues were selected and the expression levels of miR-214,RASSF5 were detected.Oral cancer cells CAL27 were cultured and divided into negative control(NC) mimc group transfected with NC mimc and miR-214 mimic group transfected with miR-214 mimic.Then cell growth viability,cell cycle distribution and expression level of cell cycle genes were detected.Results The expression level of miR-214 in oral cancer tissues was significantly higher than that of adjacent tissues,the expression level ofRASSF5 was significantly lower than that of adjacent tissues(P < 0.05) and the expression level of miR-214 was negatively correlated with the expression level of RASSF5(P < 0.05).The growth viability and Sphase,G2/M phase ratiosof oral cancer CAL27 cells in miR-214 mimic group were significantly higher than those in the NC mimic group,while theG0/G1 phase ratio was significantly lower than that in the NC mimic group and the expression levels of RASSF5,p53 and p21 in cells were significantly lower than those in NC mimic group,and the expression levels of CyclinD1 and CyclinE were significantly higher than those in NC mimic group(P < 0.05).Conclusion miR-214 can enhance the growth viability of oral cancer cell CAL27 by targeting RASSF5 gene expression.更多还原