【摘要】 目的探讨大黄酚对IgA肾病大鼠肾损伤和免疫反应的影响。方法 40只大鼠随机分为4组:对照组、大黄酚对照组(CA组)、IgA肾病模型组(IgA组)和大黄酚实验组(IgA+CA组)。IgA组和IgA+CA组自由饮溶于6m M HCl酸化水的0. 1%的牛血清球蛋白连续8周后尾静脉注射1 mg牛血清球蛋白,对照组和CA组用生理盐水代替牛血清球蛋白进行相同处理;然后CA组和IgA+CA组大鼠灌胃给予溶解于0. 9%的生理盐水的大黄酚[10 mg/(kg·d)],对照组和IgA组则给予生理盐水处理。苏木素-伊红(HE)染色观察肾脏组织病变。运用全自动生化分析仪检测大鼠尿蛋白、血清肌酸酐和尿素氮水平。蛋白印记分析大鼠细胞增殖核抗原-67(Ki-67)、增殖细胞核抗原(PCNA)、Caspase-3和Caspase-9表达。酶联免疫吸附实验检测大鼠血清中白细胞介素(IL)-1β和IL-18水平。结果与对照组相比,IgA肾病模型组大鼠尿蛋白、血清肌酸酐和尿素氮水平升高(P <0. 01)。大黄酚实验组大鼠尿蛋白、血清肌酸酐和尿素氮水平低于IgA肾病模型组(P <0. 01)。而且,IgA肾病模型组大鼠Ki67和PCNA表达低于对照组(P <0. 01)。与IgA肾病模型组相比,大黄酚实验组大鼠Ki67和PCNA表达升高(P <0. 01)。与对照组相比,IgA肾病模型组大鼠Caspase-3和Caspase-9表达增强(P <0. 01)。大黄酚实验组大鼠Caspase-3和Caspase-9表达低于IgA肾病模型组(P <0. 01)。另外,IgA肾病模型组大鼠血清中IL-1β和IL-18水平高于对照组(P <0. 01)。与IgA肾病模型组相比,大黄酚实验组大鼠血清中IL-1β和IL-18水平下降(P <0. 01)。结论大黄酚可减轻IgA肾病大鼠的肾损伤和炎症反应。更多还原

【Abstract】 Objective To explore the effect of chrysophanic acid on kidney injury and inflammatory response in IgA nephropathy rats.Methods Forty rats were randomly divided into four groups: control group,chrysophanic acid control group( CA),IgA nephropathy model group( IgA) and chrysophanic acid treatment group( IgA + CA). Pathological changes of tissues were observed by hematoxylin-eosin( HE) staining.The levels of proteinuria,serum creatinine and urea nitrogen were detected by fully automatic biochemical analyser. The expression of antigen identified by monoclonal antibody( Ki-67),proliferating cell nuclear antigen( PCNA),Caspase-3 and Caspase-9 was measured by western blot.The levels of interleukin( IL)-1β and IL-18 were tested by enzyme-linked Immunosorbent assay( ELISA). Results Compared with control group,the levels of proteinuria,serum creatinine and urea nitrogen in IgA nephropathy model group were enhanced( P < 0. 01). The levels of proteinuria,serum creatinine and urea nitrogen in chrysophanic acid treatment group were lower than IgA nephropathy model group( P < 0. 01).What’s more,the expression of Ki67 and PCNA in IgA nephropathy model group was lower than that of control group( P < 0. 01). Compared with IgA nephropathy model group,the expression of Ki67 and PCNA in chrysophanic acid treatment group was elevated( P < 0. 01). Compared with the control group,the expression of Caspase-3 and Caspase-9 in IgA nephropathy model group was higher( P < 0. 01). The expression of Caspase-3 and Caspase-9 in chrysophanic acid treatment group was lower than IgA nephropathy model group( P < 0. 01). In addition,the levels of IL-1β and IL-18 in IgA nephropathy model group were higher than those in the control group( P < 0. 01). Compared with IgA nephropathy model group,the levels of IL-1β and IL-18 in chrysophanic acid treatment group were decreased( P < 0. 01). Conclusion Chrysophanic acid alleviates the kidney injury and inflammatory response in IgA nephropathy rats.更多还原