【摘要】 目的研究精神依赖药物(海洛因)对成瘾大鼠小脑Bax基因表达的影响。方法采用剂量递增法建立成瘾大鼠模型,用酶联免疫吸附法(ELISA)检测小脑细胞Bax的基因的表达。结果连续给大鼠注射海洛因7 d后,大鼠出现明显的戒断症状;小脑细胞Bax、Caspase-3、Caspase-9水平比对照组分别升高了88. 21%、94. 21%、87. 24%,与对照组比有显著性差异(P<0. 01)。结论精神依赖药物(海洛因)对脑组织细胞的损伤作用可能通过诱导小脑Bax基因表达,激活caspase实现。更多还原

【Abstract】 Objective To study the effects of psychotropic drug( heroin) on the expression of Bax gene in the cerebellar of addicted rats. Method An addictive rat model was used to establish with a dose-increasing method and the gene expression of Bax in the cerebellar cells was detected by ELISA. Result After injecting heroin for 7 days,the rats showed obvious withdrawal symptoms. The levels of Bax,Caspase-3,and Caspase-9 in cerebellar cells were increased 88. 21%,94. 21%,and 87. 24% respectively,which was significantly different compared with the control group( P<0. 01). Conclusion The damage effect of psychoactive drugs( heroin) on brain tissue cells may be achieved by inducing the expression of cerebellar Bax gene and activating caspase.更多还原