【摘要】 目的探索滤泡辅助性T细胞(Tfh)在慢性特发性血小板减少性紫癜(ITP)患儿中的作用机制。方法用流式细胞术检测38名健康儿童、35例ITP患儿外周血CXCR5+CD4+T细胞占CD4+T细胞的比例。采用酶联免疫吸附试验检测健康儿童、ITP患儿血浆白细胞介素-21(IL-21)浓度。结果与健康对照组相比,ITP患儿外周血CXCR5+CD4+T细胞占CD4+T细胞的比例显著增高[(13.86±7.53)%,(7.65±5.36)%,P<0.05]。经过激素治疗后,ITP患儿外周血中CXCR5+CD4+T细胞占CD4+T细胞比例有所下降[(13.8±0.41)%,(8.23±6.12)%,P<0.05]。初发ITP患儿外周血血浆IL-21浓度显著高于健康对照组[(80.47±25.79)μg/mL,(32.41±17.53)μg/mL,P<0.05]。经过激素治疗后,ITP患儿外周血血浆IL-21浓度有所下降,但两者差异无统计学意义[(80.47±25.79)μg/mL,(60.35±19.54)μg/mL,P>0.05]。结论慢性ITP患儿与健康对照组相比,存在Tfh细胞数量及功能异常,可能与慢性ITP的发病相关。更多还原

【Abstract】 Objective To investigate the expression of the T follicular helper cells(Tfh) in peripheral blood of children with immune thrombocytopenic purpura(ITP). Methods Thirty-five children with ITP and 38 healthy children(control group) were enrolled in this study. The proportion of CXCR5+CD4+T cells of children with ITP and healthy controls in peripheral blood were analyzed by flow cytometry. The concentration of plasma IL-21 was determined by ELISA. Results The ratio of CXCR5+CD4+T cells in ITP children is significantly higher than that in the control group [(13.86±7.53)% vs(7.65±5.36)%, P<0.05], but decreased after the treatment of dexamethason for 30 days [(13.8±0.41)% vs(8.23±6.12)% P<0.05]. In contrast to the healthy controls, the concentration of cytokine IL-21 was significantly elevated in patients with ITP [(80.47±25.79) μg/mL vs(32.41±17.53) μg/mL P<0.05], but there is no significant change of cytokine IL-21 after the treatment of dexamethason for 30 days [(80.47±25.79) μg/mL vs(60.35±19.54) μg/mL P>0.05]. Conclusion The ratio of Tfh increased in ITP patients. Tfh might play a role in evaluation and treatment of ITP.更多还原