【摘要】 采用高效液相色谱-二极管阵列检测器法(其色谱条件:色谱柱为CAPECELL PAK MG S5 C18柱(4.6 mm×250 mm,5μm);流动相为0.1%磷酸水溶液-甲醇,其体积比为96∶4,等度洗脱;流速为1 m L/min;柱温为40℃,检测波长为210 nm)测定红香酥梨果实中6种有机酸含量。结果显示,在此色谱条件下,L-苹果酸、D-苹果酸、酒石酸、柠檬酸、琥珀酸、乳酸在20 min内均得到良好的分离,6种有机酸线性范围为0.005～2.500 mg/m L,标准曲线的相关系数均达到0.999 9,回收率为96.00%～105.42%,精密度检测RSD值为0.32%～2.64%,稳定性检测RSD值为0.92%～4.33%,重复性检测RSD值为0.66%～3.07%。表明该方法良好,可用于红香酥梨中有机酸含量的测定。更多还原

【Abstract】 Six organic acids contents were determined by HPLC with diode-array detection(DAD). The chromatographic column used is CAPECELL PAK MG S5 C18(4.6 mm×250 mm,5 μm). The mobile phase is 0.1% H3 PO4 and methanol(96∶4)solution at a flow rate of 1 mL/min. The column temperature and detection wave length are 40 ℃ and 210 nm, respectively. The results showed that under these chromatographic conditions, D(+)-malic acid, L(-)-malic acid, tartaric acid, citric acid, succinic acid and lactic acid could be successfully separated in 20 min. All the calibration curves for these organic acids displayed a good linear relationship, the linear range was 0.005-2.500 mg/m L, the correlation coefficients of the standard curve was 0.999 9 and precision RSD was 0.32%-2.64%,stability RSD was 0.92%-4.33% and repeatability RSD of 0.66%-3.07%. The recovery rates of six organic acids were in the range of96.00%-105.42%. The method is good and can be used for the determination of organic acids in red fragrant pear.更多还原