【摘要】 为建立和完善细胞在体外培养过程中清除霉菌污染的方法,挽救珍贵的细胞资源,试验在被霉菌污染的吕梁黑山羊耳缘成纤维细胞培养液中分别添加2.5,25,250μg/m L的两性霉素B,每天洗涤、换液一次,连续处理14 d,比较不同浓度药物的清除效果。结果显示,细胞培养液中添加25μg/m L的两性霉素B后,细胞中无霉菌生长,细胞生长良好,药物处理过程中可正常传代,处理后细胞生长曲线与对照组细胞无明显差异。说明细胞培养液中添加25μg/m L两性霉素B既能清除霉菌污染又不会对细胞造成影响,该浓度是清除霉菌污染适宜的质量浓度。更多还原

【Abstract】 To establish and improve the method of removing mold contamination during cell culture in vitro, and to save precious cell resources, the experiment added 2.5,25,250 μg/m L amphotericin B, respectively to the mold-cultured Lyuliang Black Goat ear fibroblast culture solution, washing and changing cell once a day, continuous treatment for 14 d, compared the removal effect of different concentrations of drugs. The results showed that after adding 25 μg/m L amphotericin B to the cell culture medium, there was no mold growth in the cells, and the cells grew well. The cells were normally passaged during the drug treatment. There was no significant difference between the cell growth curve and the control cells. Adding 25 μg/m L amphotericin B to the cell culture solution can eliminate mold contamination without affecting the cells, and this concentration is an appropriate concentration for removing mold contamination.更多还原