【摘要】 以采自江西赣州安南的野生鱼为实验材料,分别设计引物扩增其线粒体DNA COⅡ和D-loop序列并进行序列比对分析,经扩增得到的COⅡ序列全长为691 bp,D-loop序列的全长为937～944 bp,其中COⅡ序列中A、T、G、C的平均占比分别为31. 00%、25. 80%、15. 30%和27. 90%,而D-loop序列的占比则分别为35. 60%、32. 30%、12. 00%和20. 00%,两序列都是A+T含量高于G+C,G的含量在4种碱基中最低。COⅡ序列中仅存在6个碱基位点的转换,D-loop则有多个碱基位点存在转换、颠换、缺失或插入,群体内遗传距离分别为0. 0075、0. 0050。用最大似然法构建的COⅡ和D-loop系统发育树显示赣州安南光唇鱼与半刺光唇鱼(Acrossocheilus hemispinus)亲缘性最近,序列相似性分别为98. 70%、98. 69%,遗传距离最近分别为0. 0130、0. 0260,未达到种间分化的水平(0. 05)。2个序列分析结果一致,结合外部形态特征观察,可以断定赣州安南光唇鱼与半刺光唇鱼为同一种,研究为江西赣州安南光唇鱼的资源利用提供了资料。更多还原

【Abstract】 Using Acrossocheilus fasciatus from Annnan in Jiangxi province,China,as experimental materials,we analyzed the variability of mitochondrial cytochrome oxidase unit Ⅱ( COⅡ) gene and D-loop region,and discussed the phylogenetic positon and species identification of two kinds of A. fasciatus. The results showed that the COⅡ gene was 691 bp in length,and the D-loop gene was931-944 bp in length. Regardless of COII,or D-loop,the content of bases A + T was higher than that of bases G + C. Only 6 base sites were transformed in COⅡ sequence,while several base sites in D-loop sequence were transformed,transversed,and insertions/deletions. The genetic distances within the population were 0. 0075 and 0. 0050,respectively. The COⅡ and D-loop phylogenetic tree constructed by the maximum likelihood method showed the closest relationship between JXAnnan and Arossocheilus hemispinus. The sequence similarities were 98. 70% and 98. 69%,respectively. The genetic distances were 0. 0130 and 0. 0260,respectively,indicating that the genetic distance did not reach the level of interspecific divergence( 0. 05). The results demonstrated that JXAnnan is a geographical group of A. hemispinus. The results of this study can provide information for taxonomic study of Acrossocheilus.更多还原