【摘要】 采用10%盐浓度的LB培养基从玉米根际筛选并分离出一株能够耐高盐的细菌菌株,利用形态特征和16S rDNA序列对该菌进行鉴定并分析其促生特性,通过盆栽试验研究在盐胁迫下,该菌株对玉米幼苗生长的影响。研究结果表明,该菌为嗜根考克氏菌Y1,具有溶磷性;在正常条件下,将嗜根考克氏菌Y1定殖在玉米幼苗根际上,株高和根长变化量分别增加了44.96%和49.45%左右,总叶绿素含量提高了74.44%左右,而MDA的含量降低了0.65倍左右;在盐胁迫下,株高和根长变化量分别提高了1.2倍和1.17倍左右,总叶绿素含量提高了56.24%左右,CAT活性提高了0.9倍左右,而MDA含量降低了0.34倍左右。结果表明,嗜根考克氏菌Y1可以作为生物肥料应用于盐渍化土壤中,缓解盐对玉米幼苗生长的抑制,促进玉米幼苗的生长。更多还原

【Abstract】 A strain of salt-tolerant bacteria was screened and isolated from maize rhizosphere using 10% salt concentration LB medium.The strain was identified by morphological characteristics and 16 S rDNA sequence,and its growth-promoting characteristics were analyzed.The effect of the strain on maize seedling growth under salt stress was studied by pot experiment. The results showed that the strain was Kocuria rhizophila Y1 with phosphorus solubility. Under normal conditions,when K. rhizophila Y1 was colonized in the rhizosphere of maize seedlings,the plant height and root length increased by 44.96% and 49.45%,the total chlorophyll content increased by 74.44%,respectively,while the MDA content decreased by 0.65 times. Under salt stress,plant height and root length increased by 1.2 and 1.17 times,total chlorophyll content increased by 56.24%,CAT activity increased by 0.9 times,respectively,while MDA content decreased by 0.34 times. Therefore,K. rhizophila Y1 can be used as a biological fertilizer in saline soil to alleviate the inhibition of salt to the growth of maize seedlings and subsequently to promote the growth of maize seedlings.更多还原