【摘要】 旨在从龙脑樟树叶片中分离内生真菌并对其发酵产物的体外抑菌、抗肿瘤和抗氧化活性进行测定,筛选鉴定具有较强生物活性的菌株。对分离出的内生真菌,用K-b纸片法测定其抑菌活性,MTT法测定抗肿瘤活性,紫外分光光度法测定其抗氧化活性,ITS(Internal transcribed spacer)序列测序分析确定活性菌株的种属。结果显示,共从龙脑樟树叶片中分离得到内生真菌39株,其中具有对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌较强抑制活性的菌株7株。又从7株真菌中筛选出具有较强抗肿瘤活性和抗氧化活性的菌株ZS14,其对人乳腺癌细胞MCF-7抑制率达到73.00%,对DPPH清除率70.92%,对羟自由基清除率78.83%,过氧化氢清除率81.36%,据ITS测序结果将其命名为Diaporthales sp. ZS14。分离得到的菌株Diaporthales sp. ZS14具有较强的抑菌、抗肿瘤、抗氧化活性,可以作为药物开发的潜在来源进行进一步研究。更多还原

【Abstract】 This work aims to isolate endophytic fungi from leaves of Cinnamomum camphora,to determine its anti-bacterial activity,anti-tumor activity and anti-oxidation activity in vitro,and to screen and identify strains with strong biological activity. On the isolated endophytic fungi,K-b disk method was used to determine its anti-bacterial activity,MTT assay to determine its anti-tumor activity,ultraviolet spectrophotometry to determine its anti-oxidation activity,and ITS(Internal transcribed spacer)to analyze and determine the species of active strains. Results showed that total 39 endophytic fungi were isolated from the leaves of C. camphora. Among them,there were 7 strains highly inhibiting Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Pseudomonas aeruginosa. Further,the strain ZS14 with anti-tumor and anti-oxidation activities from the 7 strains was screened,its inhibition rate on human breast cancer cell MCF-7 reached 73.00%,clearance rate of DPPH was 70.92%,scavenging rate of hydroxyl radical was 78.83%,and clearance rate of hydrogen peroxide was 81.36%. With ITS sequencing results it was designated as Diaporthales sp. ZS14. In sum,the isolated strain Diaporthales sp. ZS14 had strong anti-bacterial,antitumor and anti-oxidation activities,and it can be used as potential sources for drug development in further study.更多还原