【摘要】 目的探讨2-甲氧基雌二醇（2-methoxyestradiol,2-ME）对骨肉瘤MG63细胞增殖和凋亡的影响及其分子机制。方法不同浓度（0、10、20、40μmol/L）的2-ME作用于肿瘤细胞后,通过显微镜观察细胞形态变化、MTT实验检测增殖抑制、流式细胞术检测细胞周期和凋亡、Western Blot和qRT-PCR实验检测相关分子表达情况。结果随着2-ME浓度的增加,细胞形态学观察显示肿瘤细胞数量逐渐减少、变形;MTT实验表明2-ME对肿瘤细胞增殖抑制作用逐渐增强;流式细胞术结果显示,2-ME能剂量依赖性的诱导肿瘤细胞凋亡,使肿瘤细胞周期阻滞在G₀/G₁期;Western Blot和qRT-PCR实验结果表明,细胞内Bcl-2、VEGF的表达逐渐下降,而Caspase-3的表达则逐渐升高。结论2-ME能抑制骨肉瘤MG63细胞增殖、诱导凋亡,其作用机制可能与Bcl-2、VEGF、Caspase-3相关。更多还原

【Abstract】 Objective To investigate the effects of 2-methoxyestradiol(2-ME) on proliferation and apoptosis of osteosarcoma MG63 cells and its possible molecular mechanism.Methods After treatment of tumor cells with different concentrations of 2-ME(0,10,20,40 μmol/L),cell morphology was observed by microscope,MTT assay was used to detect proliferation inhibition,cell flow cytometry was used to detect cell cycle and apoptosis,Western Blot and qRTPCR were used to detect the expression of related molecules.Results With the increase of 2-ME concentration,cell morphology observation showed that the number of tumor cells decreased and deformed gradually.MTT assay showed that2-ME inhibited the proliferation of tumor cells gradually.Flow cytometry results showed that 2-ME could induce tumor cell apoptosis in a dose-dependent manner,and the tumor cell cycle was more blocked in G0/G1 phase.The results of Western Blot and qRT-PCR showed that the expression of Bcl-2 and VEGF decreased gradually,while the expression of Caspase-3 increased gradually.Conclusion 2-ME can inhibit the proliferation and induce apoptosis of osteosarcoma MG63 cells,and its mechanism may be related to Bcl-2,VEGF and Caspase-3.更多还原