【摘要】 食品和饲料的生物催化转化和发酵技术成为保障餐桌安全及绿色发展的重要领域,其用酶量在工业酶制剂市场中占比最大,且增长最快。性能优良的酶制剂能显著缩短发酵周期,降低生产成本,提高目标产物的转化率,进而提高市场竞争力。食品和饲料加工产业除了应用分离提取的酶制剂外,很多发酵过程仍以微生物为细胞工厂进行多种酶催化转化。该发酵过程通常涉及复杂的代谢网络和调控机制,且很多体系不能采用基因工程技术,因此理性基因工程改造通常难以满足应用需求。诱变育种仍然是食品和饲料加工用酶及微生物菌株改造的主要手段,需要与时俱进不断发展新技术。近年发展起来的常压室温等离子体(ARTP)诱变育种技术因其具有操作简单、安全、环境友好、突变率高、突变库容大等特点,在食品和饲料加工微生物育种中得到了广泛应用,取得了良好的成效。重点介绍近年ARTP在食品与饲料加工酶制剂及发酵微生物改造中的相关应用进展。更多还原

【Abstract】 Biocatalytic conversion and microbial fermentation for food and feed processing are of importance. Enzymes used in food and feed fermentation account for the largest market share of total industrial enzymes and grow fastest.Enzymes with excellent performance shorten the fermentation cycle, reduce the cost and increase the productivity of the target substrates. The improvement of industrial enzymes is crucial for the product competitiveness. In addition to the application of extracted and purified enzymes, a lot of food and feed fermentation involve microorganisms as cell factory to complete the conversion and metabolism of the complex substrates catalyzed by various enzymes in the microbial cells. Due to the involvement of complex metabolic pathways and regulatory mechanisms, and the difficulty in using genetic engineering in food and feed industry, rational design and genetic engineering are usually not allowed to use in the bioprocesses. Therefore, mutagenesis is still an irreplaceable method for microbial breeding for biological processing of food and feed. Atmospheric and room temperature plasma(ARTP) mutagenesis technology developed in recent years, which has the advantages of simple operation, safe, environmental friendly and high mutation rate, can create mutation libraries with high capacity in short time and has been widely and successfully used in the microbial and enzymes improvement in food and feed industry. This article summarizes the recent progress of ARTP mutagenesis-based improvement of biocatalysts for application in food and feed industry.更多还原