【摘要】 目的:探讨早发性和迟发性阿尔茨海默病(AD)患者红细胞(RBC)膜蛋白表达的变化。方法:选取2016年1月至2018年1月经青海省人民医院确诊为AD的患者40例,根据年龄将AD患者分为迟发性AD患者(年龄≥65岁)23例和早发性AD患者(年龄65岁)17例。选取同期同医院19名年龄≥65岁的健康体检者为迟发性AD对照组,16名年龄65岁的健康体检者为早发性AD对照组。采用流式细胞术检测全血样本中葡萄糖转运蛋白1 (glucose transporter1, Glut1)、三磷酸腺苷结合盒转运蛋白A1(ATP-binding cassette transporter A1, ABCA1)、三磷酸腺苷结合盒超家族G成员2(ATP-binding cassette super-family G member 2,ABCG2)和三磷酸腺苷结合盒超家族B成员6 (ATP-binding cassette sub-family B member 6, ABCB6)等转运蛋白以及胰岛素受体(insulin receptor, INSR)、质膜钙泵(plasma membrane Ca2+ATPase, PMCA)等这些RBC膜蛋白的表达。结果:与同年龄段健康体检者对比,早发性AD患者RBC膜Glut1和INSR的表达水平显著增加(P均0.05),而ABCA1、ABCG2、PMCA和ABCB6表达无显著差异。与同年龄段健康体检者对比,迟发性AD患者RBC膜Glut1、INSR、ABCA1和ABCG2的表达显著增加(P均0.05),而PMCA和ABCB6表达无显著差异。结论:RBC膜Glut1、INSR、ABCA1和ABCG2蛋白表达的检测可能可作为AD病程诊断的新的参考标记物。更多还原

【Abstract】 Objective: To investigate the changes of membrane protein expression of red blood cell(RBC) in patients with early-onset and late-onset Alzheimer’s disease(AD). Methods: 40 patients with AD were enrolled and diagnosed by Qinghai People’s Hospital from January 2016 to January 2018. According to age, AD patients were divided into 23 patients with late-onset AD(age≥65) and 17 patients with early-onset AD(age 65). 19 healthy subjects(age≥65) were selected as the late-onset AD control group, 16 healthy subjects(age 65) were selected as early-onset AD control group in the same time and hospital. Expressions of RBC membrane proteins, such as glucose transporter1(Glut1), ATP-binding cassette transporter A1(ABCA1), ATP-binding cassette super-family G member 2(ABCG2),ATP-binding cassette sub-family B member 6(ABCB6), insulin receptor(INSR), and plasma membrane Ca2+ATPase(PMCA), were detected by flow cytometry in whole blood samples. Results: Compared with healthy subjects of the same age, expressions of Glut1 and INSR were significantly increased in RBC membrane of patients with early-onset AD(all P<0.05), while expressions of ABCA1, ABCG2,PMCA and ABCB6 had no significant differences. Compared with healthy subjects of the same age, expressions of Glut1, INSR, ABCA1 and ABCG2 were significantly increased in RBC membrane of patients with late-onset AD(all P<0.05), while there was no significant difference in the expressions of PMCA and ABCB6. Conclusions: Detection of expressions of Glut1, INSR, ABCA1 and ABCG2 protein in RBC membrane may serve as new reference markers for the diagnosis of AD disease.更多还原