【摘要】 目的:探讨免疫相关GTP酶1（Irgm 1）对小鼠血管动脉粥样硬化（AS）斑块形成的影响。方法:高脂饲料喂养野生型（WT）、ApoE^-/-Irgm 1^+/+和ApoE^-/-Irgm1^+/-小鼠3个月,建立AS模型;取小鼠主动脉弓,免疫荧光染色方法观察WT和ApoE^-/-Irgm 1^+/+小鼠血管AS斑块中Irgm 1的表达情况及部位;Western blot方法检测WT和ApoE^-/-Irgm 1^+/+小鼠血管AS斑块中Irgm 1蛋白表达情况;Q-PCR方法检测WT和ApoE^-/-Irgm 1^+/+小鼠血管AS斑块中Irgm 1 m RNA表达情况;油红O染色观察ApoE^-/-Irgm1^+/+和ApoE^-/-Irgm1^+/-小鼠血管AS斑块形成情况;结果:与WT组相比,ApoE^-/-Irgm 1^+/+组小鼠主动脉弓AS斑块中Irgm 1+细胞明显增多,Irgm 1+细胞主要位于血管AS斑块的表面;与WT组相比,ApoE^-/-Irgm 1^+/+组小鼠血管AS斑块中Irgm 1蛋白表达显著增多（P<0.001）,Irgm 1 m RNA表达显著增多（P<0.01）;与ApoE^-/-Irgm1^+/-组相比,ApoE^-/-Irgm1^+/+组小鼠主动脉弓AS斑块面积显著增大（P<0.01）;结论:Irgm 1能够促进血管AS斑块的形成。更多还原

【Abstract】 Objective: To investigate the effect of Immunity-related GTPase family M protein（Irgm1） on atherosclerotic（AS）plaque formation. Methods: Wild type（WT）, ApoE^-/-Irgm1^+/+and ApoE^-/-Irgm1^+/-mice were fed with high fat for 3 months to establish AS model. The expression of Irgm1 in WT and ApoE^-/-Irgm1^+/+AS plaques was detected by immunofluorescence, Western blot was used to detect the expression of Irgm 1 protein in AS plaques in WT and ApoE^-/-Irgm1^+/+mice, and the expression of Irgm 1 m RNA in AS plaques of these two groups was detected by Q-PCR. Finally, the formation of AS plaques in ApoE^-/-Irgm1^+/+and ApoE^-/-Irgm1^+/-mice was observed by Oil red O staining. Results: Compared with WT group, the expression of Irgm 1 in AS plaque in ApoE^-/-Irgm1^+/+mice were significantly increased（P<0.001）, and the increased Irgm 1+cells mainly concentrated on the surface of AS plaque; the Irgm1 m RNA as well as its protein were also found significantly increased（P<0.01） in ApoE^-/-Irgm1^+/+mice, compared with WT group. The AS plaque area in ApoE^-/-Irgm1^+/-group was significantly larger than that in ApoE^-/-Irgm1^+/+group（P<0.01）. Conclusion: Irgm 1 can promote the formation of vascular AS plaque.更多还原