【摘要】 研究在南瓜多糖与人参皂苷联合作用下对过氧化氢损伤后的小鼠肝细胞的抗氧化作用。取小鼠活体肝细胞进行H₂O₂诱导,成功建立细胞氧化应激损伤模型。将细胞分为正常组、模型组、不同浓度下的南瓜多糖组、人参皂苷组及联合组,考察各组分在细胞内的抗氧化活性。试验结果表明,经分离纯化的南瓜多糖具有抗氧化活性,南瓜多糖与人参皂苷Rg1联合使用时,细胞活力明显增强,在丙二醛（malondialdehyde,MDA）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）、超氧化物歧化酶（superoxide dismutase,SOD）及过氧化氢酶（catalase,CAT）指标上具有联合增效作用,抗氧化效果更加明显,缓解了氧化损伤带来的影响。更多还原

【Abstract】 The antioxidant effect of pumpkin polysaccharide and ginsenoside on the liver cells of mice after hydrogen peroxide injury was studied. In vivo mouse hepatocytes were taken for H₂ O₂ induction,and the cell oxidative stress injury model was successfully established. The cells were divided into normal group,model group,pumpkin polysaccharide group at different concentrations,ginsenoside group and combination group to investigate the antioxidant activities of each component in the cells. Experimental results showed that the separation and purification of pumpkin polysaccharide had antioxidant activity,pumpkin polysaccharide and ginseng saponin Rg1 joint, when using cell vitality enhanced obviously, than a single component in malondialdehyde（MDA）,glutathione peroxidase（GSH-Px）,superoxide dismutase（SOD）and catalase（CAT）index on the joint synergy,antioxidant effect was more obvious,and it mitigated the effects of oxidative damage.更多还原