【摘要】 为了探究胡麻粕多酚的肿瘤细胞抑制活性,本研究以胡麻粕为原材料,采用超声提取法和福林-酚法提取并检测提取物中的多酚质量浓度;利用细胞克隆等实验评价胡麻粕多酚对结肠癌以及正常肠上皮细胞增殖的抑制效应;借助流式细胞术和蛋白免疫印迹技术检测胡麻粕多酚对肠癌细胞凋亡的调控;并检测胞内活性氧(reactive oxygenspecies,ROS)水平以分析胡麻粕多酚对结肠癌细胞内ROS产生的调节作用。结果显示:胡麻粕多酚具有明显的肿瘤细胞抑制活性,并能够促进促凋亡因子Bak和Bax蛋白的表达,抑制抗凋亡因子Bcl-2和Bcl-XL蛋白的表达,且促进细胞色素c的释放;此外,随着胡麻粕多酚质量浓度的增大,DLD1和HCT-116细胞内ROS水平升高。综上所述,胡麻粕多酚能够通过提高肠癌细胞内的ROS水平并促进其进入线粒体凋亡途径从而发挥抗肿瘤作用。更多还原

【Abstract】 The objective of this study was to explore the anti-tumor activity of the polyphenols extracted from flax meal by ultrasonic-assisted extraction. The content of polyphenols in the extract was determined using the Folin-Ciocalteu assay. The clonogenic survival assay was used to evaluate the inhibitory effect of flax polyphenols on the proliferation of colon cancer and normal FHC cells. Flow cytometry was used to detect the apoptosis of fluorescence-labeled cells. Western blotting was used to detect the expression of apoptosis-related proteins. We also evaluated the regulation of intracellular reactive oxygen species(ROS) production by flax polyphenols. The results showed that flax meal polyphenols had obvious anticancer activity and pro-apoptotic activity on DLD1 and HCT-116 cells. Meanwhile, flax meal polyphenols could promote the expression of the pro-apoptotic factors Bak and Bax, inhibit the expression of the anti-apoptotic factors Bcl-2 and Bcl-XL, promote the release of cytochrome c, and elevate the level of ROS in colon cancer cells in a concentrationdependent manner. In summary, flax meal polyphenols can exert anti-tumor effects by increasing ROS level in colon cancer cells and activating mitochondrial apoptotic pathways.更多还原