【摘要】 以碱性磷酸酶、紫外吸收物、丙酮酸含量以及SDSPAGE凝胶电泳结果等为指标,通过单独及联合使用ε-聚赖氨酸盐酸盐、乳酸链球菌素(Nisin),综合评价了ε-聚赖氨酸盐酸盐、Nisin对蜡状芽孢杆菌的抑菌作用。结果表明:ε-聚赖氨酸盐酸盐与Nisin对蜡状芽孢杆菌具有协同抑制作用,Nisin主要是通过破坏细胞壁膜结构以增加细胞壁膜的通透性,而ε-聚赖氨酸盐酸盐主要是通过影响ATP酶活性、蛋白质合成与表达及能量代谢过程来影响菌体的生长代谢活动。二者联合使用,可在较低浓度下达到更好抑菌效果。更多还原

【Abstract】 The bacteriostatic action and mechanism of both against Bacillus cereus were studied by usingε-polylysine hydrochloride and nisin alone and in combination.The alkaline phosphatase content,UV absorbing content,pyruvic acid content and SDS-PAGE gel electrophoresis were used as indicators to comprehensively evaluate the antibacterial activity ofε-polylysine hydrochloride and Nisin against Bacillus cereus.The results showed thatε-polylysine hydrochloride and Nisin had a synergistic inhibitory effect on Bacillus cereus.Nisin mainly destroyed cell wall membrane structure and increased the permeability of cell wall membrane,whileε-polylysine hydrochloride mainly affected the growth and metabolism of bacteria by affecting ATPase activity,protein synthesis and expression,and energy metabolism.The combination use of theε-polylysine hydrochloride and nisin can achieve better bacteriostatic effect at lower concentrations.更多还原