【摘要】 目的建立超高效液相色谱-串联质谱法准确、快速检测玉米油中黄曲霉毒素（aflatoxins B₁、B₂、G₁、G₂）和玉米赤霉烯酮（zearalenone,ZEN）的含量,了解市售玉米油中B₁、B₂、G₁、G₂、ZEN污染状况。方法样品经乙腈:水（84:16,V:V）溶液超声离心提取后,通过多功能固相萃取柱进行净化处理,采用液相色谱-串联质谱法进行检测。结果 B₁、B₂、G₁、G₂的线性范围为0.1～20μg/L,ZEN的线性范围为3.0～200μg/L;方法回收率为74.4%～98.6%,相对标准偏差为3.9%～6.9%;B₁、B₂、G₁、G₂定量限为0.2μg/L,ZEN的定量限为6.0μg/L。市售的33份样品中,均没有检出B₁、B2、G₁、G₂;玉米赤霉烯酮检出32份,检出率为97.0%。结论该方法操作简单快速、重现性好,可用于玉米油中黄曲霉毒素和玉米赤霉烯酮的检测。更多还原

【Abstract】 Objective To establish an analysis method for accurate and rapid determination of aflatoxins（B₁,B₂,G₁,and G₂）and zearalenone（ZEN）by ultra high performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）and to elucidate the contamination situation of B₁,B₂,G₁,G₂ and ZEN in corn oil.Methods The sample was extracted by ultrasonic centrifugation of acetonitrile:water（84:16,V:V）solution,purified by multi-functional solid phase extraction column,and detected by UPLC-MS/MS.Results The linear ranges of B₁,B₂,G₁ and G₂ were 0.1-20μg/L,and the linear range of ZEN was 3.0-200μg/L.The recoveris were 74.4%-98.6%,and the relative standard deviations were 3.9%-6.9%.The limits of quantification for B₁,B₂,G₁,and G₂ were 0.2μg/L,and the limit of quantification for ZEN was 6.0μg/L.B₁,B₂,G₁,and G₂ were not detected in the samples,but ZEN were detected in 32 samples and the detection rate was 97.0%.Conclusion The method is simple,rapid,and reproducible,which can be used for the determination of aflatoxins and zearalenone in corn oil.更多还原