【摘要】 目的探讨孕期和哺乳期补硒对仔鼠海马硒蛋白mRNA表达的影响。方法从孕1 d开始给予母鼠含亚硒酸钠的饮水补硒,低、中、高3个剂量组饮水中亚硒酸钠的浓度分别为400、1600、6400μg/L,自由饮用。补硒持续至仔鼠出生后21d。采用RT-PCR测定仔鼠海马中25种硒蛋白mRNA的表达。结果仔鼠海马中25种硒蛋白mRNA均有不同程度的表达,其中SelW、SelP、GPX4、SelK15、SelK、SelM、SelT和GPX1的表达量明显优于其他硒蛋白,Dio1表达量最低;母鼠孕哺期补硒后,有15个硒蛋白基因表达量发生了不同程度的变化,高剂量组改变尤为显著; Sel15、SPS2、SelW和Txnrd2的表达相对稳定,不受饮食补硒的影响。结论孕哺期补硒能够对仔鼠海马硒蛋白的mRNA表达产生影响,进而影响仔鼠神经发育。更多还原

【Abstract】 Objective To investigate the effect of selenium supplementation during pregnancy and lactation on the expression of selenoprotein mRNA in hippocampus of progeny mouse. Methods From the first day of pregnancy, pregnancy rats were given water containing sodium selenite for free drinking. The concentration of sodium selenite in drinking water of low, medium and high dose groups were 400, 1600, 6400 μg/L, respectively.Selenium supplementation lasted 21 days after birth of offspring. The expression of selenoprotein mRNA in hippocampus of offspring was determined by RT-PCR. Results Total of 25 kinds of selenoprotein mRNA could be detected in the hippocampus of progeny mouse. The expression levels of SelW, SelP, GPX4, SelK15, SelK, SelM,SelT and GPX1 were significantly higher than other selenoproteins, and which of Dio1 was the lowest. After selenium supplementation during pregnancy and lactation, the expression of 15 selenoprotein genes changed to different degrees, especially in the high-dose group. The expression levels of Sel15, SPS2, SelW and Txnrd2 were relatively stable and not were affected by dietary supplementation. Conclusion Selenium supplementation during pregnancy and lactation can affect the expression of selenoprotein mRNA in hippocampus of progeny mouse, and then influence its neurodevelopment.更多还原