【摘要】 目的利用网络药理学研究黄芪治疗肌少-骨质疏松症的作用机制。方法首先借助TCMSP分析平台、GeneCards数据库和OMIM数据库,分别筛选中药活性成分、中药靶标、肌少症疾病靶标和骨质疏松症疾病靶标,将肌少症疾病靶标和骨质疏松症疾病靶标取交集,得到肌少-骨质疏松症疾病靶标。将中药靶标与肌少-骨质疏松症疾病靶标取交集,得到中药-疾病靶标并构建中药-疾病-靶标调控网络。然后通过构建中药-疾病共同靶标的蛋白互作网络,筛选核心靶标基因。最后将中药-疾病共同靶标进行GO功能富集分析和KEGG通路富集分析,得出中药-疾病共同靶标相关的信号通路。结果筛选得到黄芪治疗肌少-骨质疏松症的20个活性成分和10个中药-疾病共同靶标,并推断其作用机制可能与催乳素信号通路、内分泌失调信号通路、雌激素信号通路、乳腺癌信号通路、类风湿性关节炎信号通路、Toll样受体信号通路、胰岛素抵抗信号通路、肿瘤坏死因子信号通路等有关。结论基于网络药理学探讨了黄芪治疗肌少-骨质疏松症的作用靶标及信号通路,为中药单体开发提供了理论依据。更多还原

【Abstract】 Objectiv To explore the mechanism of Astragalus Membranaceus in the treatment of osteosarcopenia by network pharmacology. Methods Firstly, TCMSP analysis platform, GeneCards database and OMIM database were used to screen the active ingredients and target of Traditional Chinese Medicine(TCM), sarcopenia disease target and osteoporosis disease target. The target of sarcopenia disease and target of osteoporosis disease were intersected to obtain the target of osteosarcopenia disease. The common target of TCM-disease was obtained by intersecting TCM target and osteosarcopenia disease target, and the regulatory network of TCM-ingredient-disease-target was constructed. Then, the core target was screened by constructing the protein protein interaction(PPI) network and bar graph of TCM-disease target. Finally, GO function enrichment analysis and KEGG pathway enrichment analysis were carried out on TCMdisease target, and the signal pathway related to TCM-disease target was obtained. Results 20 active ingredients and10 TCM-disease targets were screened for the treatment of osteosarcopenia, and the mechanism may be related to prolactin signaling pathway, endocrine resistance signaling pathway, estrogen signaling pathway, breast cancer signaling pathway, rheumatoid arthritis signaling pathway, Toll-like receptor signaling pathway, insulin resistance signaling pathway and tumor necrosis factor(TNF) signaling pathway. Conclusion Based on network pharmacology, the target and signaling pathway of Astragalus Membranaceus in the treatment of osteosarcopenia was discussed, which provided a theoretical basis for the development of TCM monomer.更多还原