【摘要】 目的研究黄精不同炮制品水提物对气阴两虚模型大鼠的影响,为黄精的科学炮制及临床的合理用药提供参考。方法 69只SD大鼠随机分为正常组和模型组。采用3因素(热性药灌胃、限食、负重游泳)连续造模2周建立气阴两虚大鼠模型。42只造模成功大鼠随机分为模型组、阳性药组、市售黄精组、四制黄精组、九制黄精组。分别灌胃给予10 g/kg黄精各炮制品水提物或阳性药(生脉饮),给药6周。给药期间观察大鼠体质量、尾根部直径、面温、抓力、痛阈值、热成像等一般行为学指标。末次给药后,尾根部采血测血红蛋白(Hb);眼眶静脉取血测血谷丙转氨酶(ALT)、谷草转氨酶(AST)、葡萄糖(GLU)、三酰甘油(TG)、肌酐(Cr);ELISA法检测血清免疫球蛋白A(IgA)、免疫球蛋白M(IgM)、环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)含量。结果造模2周,与正常组比较,模型组大鼠体质量、GLU、Cr显著降低,说明造模成功。给药6周,与正常组比较,模型组各项指标(除ALT)均有显著性差异(P<0.05,P<0.01);市售黄精组尾径、GLU水平显著下降,面温显著升高(P<0.05,P<0.01);九制黄精组尾径显著下降,TG显著升高(P<0.01)。与模型组比较,黄精各炮制品均可改善气阴两虚模型大鼠的一般体征(抓力、尾根部直径、面温、热成像、痛阈值),肝功能(降低AST、ALT)及糖脂代谢(降低TG、升高GLU),增加血清IgA、IgM,调节环核苷酸系统。市售黄精对改善热成像、免疫功能、环核苷酸系统有较好的作用(P<0.05,P<0.01);四制黄精组可明显改善模型大鼠的一般体征、肝功能、糖脂代谢、免疫功能和环核苷酸系统(P<0.05,P<0.01);九制黄精可显著改善肝功能(P<0.01)。结论黄精不同炮制品均能一定程度改善气阴两虚大鼠症状,四制黄精在增加气阴两虚模型大鼠的体质量、尾径,改善模型大鼠糖脂代谢方面效果较优。更多还原

【Abstract】 Objective To study the effects of water extracts from different processed products of Rhizoma Polygonati( RP) on rat model of qi and yin deficiency,and to provide reference for scientific processing and clinical rational use of the drug. Methods 69 SD rats were randomly divided into normal group and model group. The rat model of qi and yin deficiency was established considering three factors( gavage administration of medicine hot in property,food restriction and weight-bearing swimming) for 2 weeks. 42 rats with successful modeling were randomly divided into model group,positive group,commercial RP group,four-fold processed RP group and nine-fold processed RP group. Each group was given 10 g/kg of water extracts from different processed products or positive drug( Shengmai Drink) for 6 weeks. The general behavioral indicators were observed during administration,including body mass,diameter of tail root,face temperature,grip strength,pain threshold and thermal imaging. After last administration,blood samples were collected from tail root to determine hemoglobin; and blood samples were collected from orbital vein to determine alanine aminotransferase(ALT),aspartate aminotransferase( AST), glucose( GLU), triglyceride( TG) and creatinine( Cr) in blood,and to determine immunoglobulin A( IgA),immunoglobulin M( IgM),adenosine cyclic phosphate( c AMP) and guanosine cyclic phosphate( c GMP) in serum by ELISA. Results After 2 weeks of modeling,compared with the normal group,there was significant decrease in body mass,GLU,and Cr in the model group,indicating successful modelling. After 6 weeks of administration,compared with the normal group,the indicators( except ALT) were significantly different in the model group( P<0.05,P<0.01); there were significant decrease in tail diameter and GLU and increase in face temperature in the commercial RP group( P<0.05,P<0.01); there were significant decrease in tail diameter and increase in TG in the nine-fold RP group( P<0.01). Compared with the model group,all processed products of RP could improve the general signs( grip strength,diameter of tail root,face temperature,thermal imaging and pain threshold),liver function( decrease in AST and ALT) and glycolipid metabolism( decrease in TG and increase in GLU),increase serum IgA and IgM,and regulate the cyclic nucleotide system of rats with qi and yin deficiency. Commercial RP had desirable effect in improving thermal imaging,immune function and the cyclic nucleotide system( P < 0. 05,P < 0. 01). Four-fold processed RP had statistical significance in improving general signs,liver function,glycolipid metabolism,immune function and the cyclic nucleotide system( P< 0.05,P<0.01). Nine-fold processed RP significantly improved liver function( P< 0.01). Conclusion Different processed products of RP can improve the symptoms of qi and yin deficiency in rats. Four-fold processed RP has better effect in increasing body mass and tail diameter of the rats with qi and yin deficiency,and it is better at regulating glycolipid metabolism in rats.更多还原