【摘要】 以目前上海市主栽的高产常规水稻"秀水134"为材料,利用CRISPR/Cas9技术成功敲除甜菜碱醛脱氢酶2基因,获得了两种类型纯合突变体植株.采用表达载体特异性结合的引物检测T₁代转基因植株,成功获得6株不携带载体骨架的转基因植株.定量PCR分析显示,突变体植株甜菜碱醛脱氢酶2基因表达量极显著低于野生型对照（p<0.01）,但突变体植株成熟种子香味物质2-乙酰-1-吡咯啉（2AP）含量极显著高于野生型对照（p<0.01）.比较野生型对照与突变体植株的主要农艺性状和产量性状,两者间都没有显著差异（p>0.05）.本研究可为加快高产香型水稻在上海及周边地区的推广应用,以及为今后利用CRISPR/Cas9技术快速培育其他高产香型水稻新品种研究奠定基础.更多还原

【Abstract】 In the study,fragrance rice mutant lines were developed with CRISPR/CAS9 by editing fragrance gene Badh2 in "Xiushui 134",a major elite rice cultivar in Shanghai region.Six mutant lines without the vector backbone were successfully obtained by PCR detection.Quantitative PCR analysis showed that the expression of Badh2 in mutant plants was significantly lower than that in wild type(p<0.01),the content of 2 AP in mature seed of mutant plants was significantly higher than that in wild type(p<0.01),but there were no significant differences on main agronomic traits between the mutant plants and wild type(p>0.05).This research can accelerate the application of high-yield fragrant rice in Shanghai areas,and lay the foundation for the future study on the rapid cultivation of other high-yield fragrant rice varieties using CRISPR/Cas9 technology.更多还原