【摘要】 目的观察口服黑升麻提取物对围绝经期雌鼠糖脂代谢的调节作用。方法选取30只处于围绝经期的雌鼠随机分为3组,分别记为黑升麻组、雌激素组和对照组,另选取6月龄SD雌鼠10只,记为青年对照组。黑升麻组雌鼠每日口服黑升麻提取物60 mg/kg,雌激素组雌鼠每日口服雌激素0.8 mg/kg,对照组和青年对照组雌鼠每日口服5%生理盐水10 mL/kg。各组雌鼠在入组时(T0)及治疗2个月后(T1),称量大鼠体质量;采集鼠尾静脉血,检测糖脂代谢指标:应用全自动生化分析仪检测血清空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG),采用ELISA法检测血清空腹胰岛素(FINS)、8点皮质醇、超敏C反应蛋白(hs-CRP),计算胰岛素敏感性指数(ISI,ISI=1/[Log(FINS)+Log(FBG)])及胰岛素抵抗指数(HOMA-IR,HOMA-IR=FBG×FINS/22.5)。结果与青年对照组相比,对照组雌鼠在T1时体质量、FBG、FINS、HOMA-IR、TC、TG、8点皮质醇、hs-CRP均升高(P均<0.05),ISI降低(P<0.05)。与对照组相比,黑升麻组雌鼠在T1时体质量、FBG、FINS、HOMA-IR、TG、8点皮质醇、hs-CRP均降低(P均<0.05),ISI升高(P<0.05);雌激素组雌鼠在T1时体质量、FBG、TG、hs-CRP均降低(P均<0.05)。与雌激素组相比,黑升麻组雌鼠在T1时FINS、HOMA-IR、8点皮质醇均降低(P均<0.05),ISI升高(P<0.05)。结论围绝经期雌鼠糖脂代谢指标紊乱;黑升麻提取物具有调节围绝经期雌鼠糖脂代谢指标的作用;与雌激素相比,黑升麻提取物可有效缓解围绝经期雌鼠的8点皮质醇水平及HOMA-IR。更多还原

【Abstract】 Objective To observe the effect of black cohosh extract on glucose and lipid metabolism in perimenopausal female rats. Methods Thirty perimenopausal female rats were randomly divided into three groups: black cohosh group, estrogen group, and control group. Another ten 6-month-old SD female rats were selected and recorded as the young control group. The female rats in the black cohosh group took 60 mg/kg black cohosh extract daily, the female rats in the estrogen group took 0.8 mg/kg estrogen daily, and the female rats in the control group and the young control group took 5% normal saline 10 mL/kg daily. At the time of entering groups(T0) and 2 months after treatment(T1), the body weight of female rats in each group was weighed; the tail vein blood of rats was collected and the metabolic indexes of glucose and lipid were detected: FBG, TC and TG were detected by automatic biochemical analyzer; FINS, 8-point cortisol and hs-CRP were detected by ELISA; and we calculated insulin sensitivity index(ISI, ISI=1/[Log(FINS)+Log(FBG)]) and insulin resistance index(HOMA-IR, HOMA-IR=FBG×FINS/22.5). Results Compared with the young control group, the body weight, FBG, FINS, HOMA-IR, TC, TG, 8-point cortisol, hs-CRP of female rats in the control group increased more(P<0.05), ISI decreased more(P<0.05). Compared with the control group, the body weight, FBG, FINS, HOMA-IR, TG, 8-point cortisol and hs-CRP of the female rats in the black cohosh group decreased(all P<0.05), ISI increased(P<0.05); the body weight, FBG, TG and hs-CRP of the female rats in the estrogen group decreased(all P<0.05). Compared with the estrogen group, FINS, HOMA-IR and 8-point cortisol in the black cohosh group decreased(all P<0.05), and ISI increased(P<0.05). Conclusions Perimenopausal female rats have disordered glucose and lipid metabolism indicators; black cohosh extract can regulate the glycolipid metabolism of perimenopausal female rats; compared with estrogen, black cohosh extract can effectively alleviate 8-point cortisol level and HOMA-IR of perimenopausal female rats.更多还原