【摘要】 目的观察自拟放射性肠炎灌肠液对急性放射性肠炎小鼠肠道黏膜屏障损伤的预防及治疗作用。方法取C57BL/6J小鼠64只,随机分为对照组、照射组、预防组和治疗组,每组各16只。对照组不予照射,照射组、预防组和治疗组接受腹部局部12 Gy照射制备急性放射性肠炎模型。预防组在造模前3天起用自拟放射性肠炎灌肠液灌肠,1次/d,造模后继续灌肠4 d,共灌肠7次;治疗组在造模当日用自拟放射性肠炎灌肠液灌肠,1次/d,共灌肠7次;对照组、照射组自造模当日均予等量生理盐水灌肠,共灌肠7次。观察各组小鼠造模15 d时各组小鼠生存情况。造模第3天时各组各取6只小鼠,颈椎脱臼处死,取小肠组织,HE染色后测算各组小肠黏膜隐窝细胞数,免疫组化Villin染色后观察小肠绒毛形态、测算小肠绒毛长度。造模第15天时观察各组另10小鼠生存情况。结果预防组、治疗组、对照组及照射组小肠组织隐窝细胞数分别为(10.30±2.21)、(3.40±2.91)、(16.90±5.55)、(6.90±3.51)个/cm,与对照组比较,照射组、预防组和治疗组小鼠小肠组织隐窝细胞数降低(P均<0.05),与照射组比较,预防组小鼠小肠组织隐窝细胞数升高,治疗组小肠组织隐窝细胞数降低(P均<0.05)。预防组、治疗组、对照组及照射组小肠组织绒毛长度分别为(386.43±56.48)、(189.42±39.90)、(585.32±21.39)、(227.22±44.36)μm,与对照组比较,照射组、预防组和治疗组小肠组织绒毛长度降低(P均<0.05),与照射组比较,预防组小肠组织绒毛长度升高,(P均<0.05)。造模第15天时,预防组、治疗组、对照组及照射组小鼠分别存活5、4、10、3只,与对照组比较,照射组、预防组和治疗组小鼠存活只数少(P均<0.05),与照射组比较,预防组和治疗组小鼠存活只数均升高(P均<0.05)。结论自拟放射性肠炎灌肠液预灌肠的急性放射性肠炎小鼠肠道黏膜屏障损伤程度低,发生急性放射性肠炎的小鼠自拟放射性肠炎灌肠液灌肠后肠道黏膜屏障损伤程度减轻。自拟放射性肠炎灌肠液灌肠对急性放射性肠炎小鼠肠道黏膜屏障损伤有预防及治疗作用。更多还原

【Abstract】 Objective To investigate the prevention and therapeutic effects of self-made traditional Chinese medicine enema(radiation enteritis enema) on intestinal mucosal barrier injury in mice with acute radiation enteritis. Methods The C57BL/6J mice were randomly divided into the control group, irradiation group, prevention group, and treatment group, with 10 mice in each group. No radiation was given to the control group; the mice in the irradiation group, the prevention group and the treatment group received 12 Gy irradiation to prepare the acute radiation enteritis models. The self-designed radioactive enteritis enemas were given 3 days before irradiation in the prevention group, once a day, for 4 days, and 7 times in total. On the day of modeling, the mice in the treatment group received enema, once a day, a total of 7 times; the mice in the control group and the irradiation group were given the same amount of normal saline enema. We observed survival of mice in each group at 15 d after modeling, observed the small intestine by Image J 1.37 analysis software, and measured the number of cryst cells and villi length.Results The number of intestinal tissue crypt cells in the prevention group, treatment group, control group and irradiation group was(10.3±2.21),(3.40±2.91),(16.9±5.55) and(6.9±3.51)/cm, respectively. Compared with the control group, the irradiation group, prevention group and treatment group decreased(P<0.05); compared with the irradiation group, the the prevention group decreased and treatment group decreased(P<0.05). It indicated that the radiation could damage the intestinal mucosal barrier of mice, and the enema given before irradiation had a preventive and protective effect on the intestinal mucosa, while the enema given after radiation the intestinal mucosal barrier was seriously damaged, with a poor effect. The villus length(μm) of small intestine in the prevention group, treatment group, control group, and irradiation group was 386.43±56.48, 189.42±39.90, 585.32±21.39, and 227.22±44.36, respectively. Compared with the control group, the irradiation group, prevention group and treatment group decreased(P<0.05); compared with the irradiation group, the the prevention group decreased(P<0.05). After 15 days of modeling, 10, 3, 5 and 4 mice in the control group, irradiation group, prevention group and treatment group survived. Compared with the control group, the survival rates in the irradiation group, prevention group and treatment group decreased(all P<0.05); compared with the irradiation group, the survival rates in the prevention group and treatment group decreased(both P<0.05).Conclusion The self-designed radioactive enteritis enema can reduce and slow the intestinal mucosal barrier injury in mice with acute radiation enteritis, and has preventive and therapeutic effects on the intestinal mucosal barrier injury in mice.更多还原