【摘要】 建立蜜炙款冬花的质量标准。方法:采用薄层色谱法对蜜炙款冬花进行定性鉴别。采用高效液相色谱法对蜜炙款冬花中的款冬酮含量进行定量分析,色谱条件以十八烷基硅烷键合硅胶为填充剂,以甲醇∶水（85∶15）为流动相,检测波长为220 nm。另外,水分以及醇溶性浸出物参照2015版《中国药典》进行测定。结果:在薄层板色谱上,供试品薄层色谱在对照药材色谱与对照品色谱相应的位置出现相同颜色的斑点;水分不得超过14%,总灰分不得超过14%,醇溶性浸出物不得少于19%;供试品按干燥品计算,含款冬酮(C₂₃H₃₄O₅)不得低于0. 12%。结论:本研究拟订的质量标准可应用于蜜炙款冬花的质量控制。更多还原

【Abstract】 A method was established to set up evaluation standard for honey-fried tussilago farfara. The qualitative identification of honey-fried tussilago farfara was performed with thin layer chromatography. Quantitative analysis of tussilagone in honey-fried tussilago farfara was carried out based on high performance liquid chromatography. The chromatographic conditions were octadecylsilane-bonded silica gel as the filler and methanol-water( 85 ∶ 15) as the mobile phase. The detection wavelength was 220 nm. In addition,moisture content and alcohol-soluble extracts were measured with reference to the 2015 Chinese Pharmacopoeia. The experiment results show that the samples have the same color spots with control article and reference crude herb in corresponding chromatography location. The honey-fried tussilago farfara moisture content is less than 14%,and total ashes content must not more than 14%,and the extract not less than 19. 0%,and the content of tussilagone not less than 0. 12%. It can be concluded that the method is simple,reproducible and stable.更多还原