【摘要】 为通过荧光免疫层析技术制备一种快速、灵敏、特异性强的尿液中苯乙醇胺A(phenylethanolamine A,PEAA)的检测方法,采用重氮化法合成PEAA人工抗原,并通过免疫、融合、有限稀释法获得特异性抗PEAA的单克隆抗体,通过将量子点微球与PEAA偶联,制备荧光免疫层析试纸条。结果表明:PEAA免疫荧光层析试纸条的检测限为0.496μg/L,回收率在80%~120%之间,批内、批间变异系数均小于10%,与其他类似物的交叉反应率小于5%,特异性良好。说明将荧光免疫层析技术应用到PEAA检测中是可行的,且制备的PEAA荧光免疫层析试纸条灵敏度高、准确性好、特异性好,应用前景广阔。更多还原

【Abstract】 A rapid, sensitive and specific immunofluorescence method to detect phenylethanolamine A(PEAA) in urine from farm animals was developed. The diazotization method was used to conjugate PEAA to bovine serum albumin(BSA),yielding an artificial antigen. Furthermore, a specific anti-PEAA monoclonal antibody was obtained by immunization, fusion,and limiting dilution analysis. Finally, a fluorescent immunochromatographic test strip was prepared by coupling quantum dot microspheres with PEAA. The results showed that the detection limit of the test strip was 0.496 μg/L. The recovery was between 80% and 120%, and the inter and intra-batch coefficients of variation(CVs) were both less than 10%. The crossreactivity with other analogues was less than 5%. The specificity was good. Accordingly, the immunofluorescence assay was feasible for the detection of PEAA with good sensitivity, accuracy and specificity and could have wide application prospects.更多还原