【摘要】 【目的】采用网络药理学预测丹参治疗糖尿病肾病可能的作用机制。【方法】应用中药系统药理学数据库和分析平台(TCMSP)挖掘单味中药丹参的入血活性成分,检索与疾病靶点有关的数据库TTD、DrugBank、OMIM、GAD及Pharm GKB筛选糖尿病肾病的作用靶点,再基于生物信息学手段拓扑丹参防治糖尿病肾病的特异性靶点,然后采用ClueGO针对关键靶点进一步分析其可能的作用机制。【结果】通过中药系统药理学数据库和分析平台挖掘出202个丹参相关成分,根据OB和DL等参数筛选出65个入血活性成分,预测出103个可能的作用靶点;通过TTD、DrugBank、OMIM、GAD及Pharm GKB等数据库检索到113个与糖尿病肾病相关的已知靶点。利用Cytoscape软件拓扑分析筛选出112个关键靶点;基于上述靶点可知,丹参作用于糖尿病肾病的核心非广泛意义的信号通路共26条。【结论】丹参治疗糖尿病肾病具有多成分、多靶点的特点,可能与细胞增殖、分化及凋亡相关,通过调节糖脂代谢、炎症反应及氧化应激等保护肾小球滤过屏障,减轻肾损伤以达到延缓疾病发生发展的目的。更多还原

【Abstract】 Objective To predict the possible therapeutic mechanism of Radix Salviae Miltiorrhizae(RSM)for thetreatment of diabetic nephropathy(DN)by network pharmacology. Methods The blood active constituents fromsingle Chinese Medicine RSM were mined from Traditional Chinese Medicine Systems Pharmacology Database andAnalysis Platform(TCMSP),and the action targets related with DN were screened from disease-associated dataincluding TTD,DrugBank,OMIM,GAD and Pharm GKB. By using bioinformatics methods,the specific targetsof diabetic nephropathy prevented and treated by RSM were explored. And then ClueGo was used to further analyzeits molecular mechanism. Results A total of 202 constituents from RSM were retrieved in TCMSP. According to theparameters such as OB and DL,65 blood active components were screened out,and 103 potential targets werepredicted out. A total of 113 known targets closely related with diabetic nephropathy were retrieved in the databaseof TTD,DrugBank,OMIM,GAD and Pharm GKB. A total of 112 key targets were screened out by Cytoscapesoftwork topological analysis. Based on the above targets, 26 key but non-extensively significant signalingpathways involving the actions of RSM on DN were found. Conclusion RSM has the characteristics ofmulticomponents and multiple targets for the treatment of diabetic nephropathy, which are involved in cellproliferation, differentiation and apoptosis. RSM can regulate glucose and lipid metabolism, inflammatoryreaction and oxidative stress to reduce renal injury and protect glomerular filtration barrier, thus to delay theprogress of DN.更多还原