【摘要】 通过使用二茂铁（Fc）功能化的还原氧化石墨烯（RGO）复合材料及核酸扩增技术构建了一个免标记且简易的电化学生物传感器,实现对食源性弧菌的高灵敏度地检测。探索了最佳的实验体条件,在最佳条件下,二茂铁的峰电流信号的改变与目标物的浓度有良好的线性关系,线性范围为10^-22～10^-15 mol·L^-1,检测限为4.6×10^-23 mol·L^-1,该电化学生物传感器在生物分析和临床诊断领域具有潜在的应用价值。更多还原

【Abstract】 A label-free and simple electrochemical biosensorwas constructedthat enables highly sensitive detection of Vibrio food-producing bacteria by using ferrocene（Fc） functionalized reduced graphene oxide（RGO） composites and nucleic acid amplification techniques. As a good electron transfer medium, ferrocene is prepared by a non-covalent bond with redox graphene to form a composite material. Double-stranded（dsDNA） of Vibrio, the product of the amplification technique, combined with composite material by π-π electrostatic action. Due to it hinders the transfer of electrons, the response of the ferrocene as a signal molecule is greatly reduced, thus the target can be detected sensitively. Because DNA rarely exists in a single-stranded form in nature, most DNA and PCR amplification products are also double-stranded, so detecting dsDNA has important practical significance.In this experiment, the optimal experimental conditions were explored. Under the optimal conditions, the peak current signal of ferrocene has a good linear relationship with the concentration of the target. The linear range is 10^-22—10^-15 mol ·L^-1. The limit is 4.6 × 10^-23 mol ·L^-1. The electrochemical biosensor has potential application value in the fields of biological analysis and clinical diagnosis.更多还原