【摘要】 目的探讨1-磷酸鞘氨醇受体3(S1PR3)在Ⅰ型糖尿病所致勃起功能障碍(ED)大鼠阴茎海绵体组织中的表达,为糖尿病ED的机制研究提供依据。方法 18只正常8周龄雄性SD大鼠随机分为正常对照组和糖尿病组,建模8周后,测定2组大鼠阴茎海绵体内压/平均动脉压,采用免疫组化、PCR技术和Western blot方法检测S1PR3在2组大鼠阴茎海绵体组织中的表达水平,使用Western blot检测2组大鼠阴茎海绵体组织中RhoA、ROCK1和ROCK2的表达水平。结果与正常对照组相比,糖尿病组大鼠勃起功能明显降低(P<0.05);糖尿病大鼠阴茎海绵体组织中S1PR3mRNA及其蛋白、RhoA、ROCK1和ROCK2蛋白的表达水平均明显升高(P<0.05)。结论Ⅰ型糖尿病可诱导大鼠ED,其原因可能与大鼠阴茎海绵体组织中S1PR3表达上调,进而激活RhoA/Rho激酶通路有关。更多还原

【Abstract】 Objective To investigate the mechanism of sphingosine-1-phosphate receptor 3(S1 PR3)in the cavernous tissues of diabetes mellitus-induced erection dysfunction(ED)rats. Methods Eighteen eight-week-old healthy male SD rats were randomly divided into 2 groups:Control group(CO,n=6);Diabetes mellitus group(DM,n=12).Diabetes rats were constructed,after 8 weeks,the maximum intracavernous pressure/mean arterial pressure were determined and the expression of S1 PR3 were detected in the penis by IHC,PCR and Western blot,and the expression of RhoA,ROCK1 and ROCK2 in rat penile cavernous tissue were detected by Western blot. Results The erectile function significantly decreased in DM rats,compared to that of age-matched control rats(P<0.05);the expression level of S1 PR3,RhoA,ROCK1 and ROCK2 were higher in DM group than those in CO(P<0.05). Conclusions TypeⅠ DM can induce ED in rats,which may be related to the up-regulation of S1 PR3 expression and the activation of RhoA/Rho kinase pathway in rat penile cavernous tissue.更多还原