【摘要】 以活性干酵母为原料,采用超声波破碎细胞,通过热变性及丙酮沉淀对活性干酵母中的超氧化物歧化酶进行分离提取.结果表明:在超声功率427 W,超声时间8 min,时间间隔2 s/7 s,热变性温度60℃,提取时间为25 min,采用0.6倍体积丙酮沉淀条件下,所获得的SOD比活力相对最高,比活力为403.53 U/mg·pro.更多还原

【Abstract】 The superoxide dismutase(SOD) in active dry yeast is extracted by thermal denaturation and acetone precipitation.The results showed that under the conditions of ultrasonic power 427 W, ultrasonic time 8 min, time interval 2 s/7 s, thermal denaturation temperature 60 ℃, extraction time 25 min, and acetone precipitation of 0.6 times volume, SOD specific activity is relatively the highest, 403.53 U/mg·pro.更多还原