【摘要】 虹鳟(Oncorhynchus mykiss)作为典型的冷水性鱼类,对较高水温的耐受能力差,随着全球气候变暖,夏季高温对其养殖的威胁越来越严重。为了进一步探寻热休克蛋白(heat shock proteins, Hsps)在虹鳟热应激过程中的作用及其机理,本研究通过对虹鳟基因组数据库和RNA-seq数据进行生物信息学分析,共鉴定出36个Hsp40家族基因,并在全基因组水平对虹鳟Hsp40家族基因进行了理化性质、系统发育、基因结构及基因水平表达谱的系统分析。结果显示,根据结构的相似性这些基因可分为3种不同的亚型:Ⅰ、Ⅱ和Ⅲ,编码氨基酸190～760个,理论等电点5.01～9.09;基因结构分析显示,虹鳟Hsp40家族基因都只有1个外显子;亚细胞定位分析表明,虹鳟Hsp40家族基因主要在细胞质、细胞核和线粒体中表达;二级结构主要以α-螺旋和不规则卷曲为主。通过RNA-seq数据对虹鳟Hsp40表达谱分析显示,虹鳟热应激后有8个Hsp40基因在肝脏中表达上调,10个基因在头肾中表达上调,在两个组织中均表达且上调的基因有7个。热应激后Hsp40家族基因的显著表达调控表明Hsp40家族基因参与了虹鳟热应激,该结果为研究Hsp40家族基因成员抗热应激的调控机制提供基础数据。更多还原

【Abstract】 Rainbow trout(Oncorhynchus mykiss) is a typical cold-water fish, which has poor tolerance to higher water temperature. With the global warming, the impact of heat stress on rainbow trout is becoming more and more serious. To further understand the mechanism of heat shock proteins(Hsps) in rainbow trout during heat stress, in this study, 36 Hsp40 family genes were identified by bioinformatics analysis of rainbow trout genome database and RNA-seq data. The physiochemical properties, Phylogenetic, gene structure and gene expression profile of rainbow trout Hsp40 family gene were systematically analyzed at the whole gene level. The results showed that these genes were divided into three subtypes Ⅰ, Ⅱ and Ⅲ according to their structural similarities. The number of amino acid ranged from 190 to 760, and the theoretical p I distribution ranged from 5.01 to 9.09. Gene structure analysis showed that the Hsp40 family genes of rainbow trout had only 1 exon. Subcellular localization analysis showed that the Hsp40 gene was mainly expressed in cytoplasm,nucleus and mitochondria, and the secondary structure was mainly alpha-helix and irregular curl. The expression profile of Hsp40 in rainbow trout was analyzed by RNA-seq data. The results showed that 8 and 10 Hsp40 genes were up-regulated in liver and head kidney, respectively, and in total of 7 Hsp40 genes were upregulated in both tissues. The significant expression regulation of Hsp40 family genes after heat stress indicates that Hsp40 genes are involved in the heat stress of rainbow trout, which provides theoretical basis and basic data for studying the regulation mechanism of Hsp40 family gene members against heat stress.更多还原