【摘要】 鸭(Anas platyrhynchos domestica)腺病毒3型(Duck adenovirus type 3, DAdV-3)是近年来我国新发现的鸭源腺病毒。本研究根据NCBI数据库中DAdV-3代表株(CH-GD-12-2014株, GenBank No. KR135164)的22K基因特征,设计特异性引物和探针,建立检测DAdV-3的MGB TaqMan探针qRT-PCR方法。结果表明,建立的qRT-PCR方法具有如下特性:灵敏度高,最低检测限为55拷贝/μL;特异性强,和鸭群常见传染病病原均无交叉反应;重复性好,组内和组间重复实验变异系数分别为0.44%～2.10%和0.64%～2.37%。利用建立的qRT-PCR方法对临床送检的61份鸭源病料进行检测,检出DAdV-3阳性15份,阳性率为24.6%。本研究为后续开展DAdV-3感染的实验室快速诊断和流行病学调查奠定了初步基础。更多还原

【Abstract】 Duck(Anas platyrhynchos domestica) adenovirus type 3(DAdV-3) is a newly discovered duckderived adenovirus in recent years. In this study, specific primers and probe were designed based on the 22 K gene characteristics of DAdV-3 representative strain(CH-GD-12-2014 strain, GenBank No. KR135164) in the NCBI database, and a MGB TaqMan probe real-time quantitative PCR method for detecting DAdV-3 was established. The results showed that the established q RT-PCR method had the following characteristics: High sensitivity, minimum detection limit of 55 copies/μL; Strong specificity, and no cross-reaction with common infectious disease pathogens in duck flocks; Good reproducibility, intra-group and inter-group repetitive experimental variation coefficients were 0.44%～2.10% and 0.64%～2.37%, respectively. 61 duck-derived materials were detected by the established method, and 15 positive DAdV-3 samples were detected with a positive rate of 24.6%. This study laid the foundation for rapid laboratory diagnosis and epidemiological investigation of DAdV-3 infection.更多还原