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福建漳州番茄黄化曲叶病病原分子诊断及暴发成因分析

Pathogeny Molecular Diagnosis and Outbreak Factor Analysis of Tomato Yellow Leaf Curl Disease in Zhangzhou,Fujian Province

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【作者】 郑宇丁雪玲姚凤銮卢学松何玉仙翁启勇

【Author】 ZHENG Yu;DING Xue-Lin;YAO Feng-Luan;LU Xue-Song;HE Yu-Xian;WENG Qi-Yong;Fuzhou Scientific Observing and Experimental Station of Crop Pests of Ministry of Agriculture, Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences;State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University;

【通讯作者】 何玉仙;

【机构】 农业部福州作物有害生物科学观测实验站福建省作物有害生物监测与治理重点实验室福建省农业科学院植物保护研究所福建农林大学闽台作物有害生物生态防控国家重点实验室

【摘要】 2017年9月福建漳州地区龙海市东园乡番茄(Solanum lycopersicum)种植区暴发病毒病害,导致严重减产,同时病区内烟粉虱(Bemisia tabaci)虫害发生严重。为明确该地区番茄病毒病突然成灾原因,本研究采用分子生物学方法对番茄病毒病原、病区内烟粉虱的生物型以及虫体带毒情况进行分子检测,并通过室内接毒实验对烟粉虱的传毒性和传毒能力进行测定。结果表明,漳州番茄病毒病原为番茄黄化曲叶病毒(Tomato yellow leaf curl virus, TYLCV);该病区采集的烟粉虱为B生物型,B型烟粉虱具有极强的获毒和传毒能力;虫体对TYLCV病毒携带率为100%。本研究证实了外来入侵烟粉虱在TYLCV病毒传播中的重要媒介作用,揭示了福建省番茄种植区病毒暴发成灾的主要原因,这为将来该病毒的防治提供了依据和帮助。

【Abstract】 In September of 2017, a serious virus disease of tomato(Solanum lycopersicum) broke out in Dongyuan town, Zhangzhou city, Fujian province, which caused severe yield loss and accompanied with the occurrence of a large number of whiteflies(Bemisia tabaci). In order to identify what type of the pathogenic virus, molecular diagnosis of the virus strain isolates was done in this research, and then, the biotype and the virus carrying rate of B. tabaci were surveyed. At the same time, the acquisition, transmission and retention of gemini viruses by B. tabaci were studied in laboratory. The results revealed that the virus sample was Tomato yellow leaf curl virus(TYLCV), the B. tabaci was B-biotype and had a very strong ability to acquire and transmit the TYLCV. The virus-carrying rate could reach up to 100%. All this indicated that whiteflytransmitted gemini viruses(WTG) was a main reason to cause the outbreak of TYLCV disease on tomato in Zhangzhou city. This will provide the basis and help for the prevention and control of TYLCV in the future.

【基金】 福建省公益类科研院所专项(No.2015R1024-4和No.2017R1025-1);福建省科技重大专项(No.2017NZ0003-1)
  • 【文献出处】 农业生物技术学报 ,Journal of Agricultural Biotechnology , 编辑部邮箱 ,2019年02期
  • 【分类号】S436.412
  • 【被引频次】4
  • 【下载频次】166
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