【摘要】 为了揭示表面活性蛋白SP-A和SP-D分泌细胞在呼吸系统中的分布.使用大鼠鼻腔到肺的各部位组织冰冻切片,进行免疫组织化学染色.结果显示,从鼻腔到小支气管的小颗粒细胞和杯状细胞细呈SP-D阳性;在喉腺和气管腺及其导管上皮呈SP-D强阳性;在细支气管克拉拉细胞和肺Ⅱ型细胞均呈SP-A和SP-D阳性.揭示了在大鼠呼吸系统中SP-D分泌细胞比SP-A分泌细胞更广泛分布,SP-A与SP-D对于维持肺部无菌环境起到重要免疫防御作用.更多还原

【Abstract】 To study the distribution of the surfactant proteins A and D secreting cells in rat respiratory system,tissues from nasal cavity to lungs were sampled and made into frozen sections for immunohistochemical(IHC) staining.The results demonstrated that,SP-D was positive stained in small granulosa cells and goblet cells from the nasal cavity to the small bronchus and positive stained in the larynx and trachea.The Laryngeal gland and tracheal gland and its ductal epithelium were strongly positive stained for SP-D.SP-A and SP-D were positive stained in Bronchial Clara cell and lung type II cells.In conclusion,SP-D secreting cells distributed more widely than SP-A secreting cells in rat respiratory system and they may played important roles in the immune and defense in the lung to keep an aseptic environment.更多还原