【摘要】 蚜虫是一种常见的农作物害虫,作为多种植物病毒的载体而成为影响农业发展的重要害虫之一,对蚜虫的防治一直是众多科学工作者所面对的难题。构建了雪花莲凝集素(GNA)和苋菜凝集素(ACA)双价植物表达载体pBI121-GA,在农杆菌介导下,叶盘法转化烟草NC89,得到卡那霉素抗性植株65株。通过基因组PCR、ELISA、RT-PCR和Western-blot等分子生物学检测,从中筛选出外源凝集素不同表达水平的转基因烟草5株。对筛选出的5株转基因烟草植株进行初步蚜虫抗性实验,证实转基因烟草对桃蚜表现出一定的抗性,最高抑制率达85.8%,为农作物双价抗蚜基因的应用研究提供一定的理论基础。更多还原

【Abstract】 Aphid is a common crop pest. As a kind of agricultural pest and the carrier of plant viruses,aphid results in serious damage in the development of agriculture and its control has always been a difficult problem faced by many scientists. A bivalent plant expression vector PB1121-GA containing the agglutinin genes of Glanthus nivalis and Amaranthus caudatus was constructed and transformed into tobacco by using Agrobacterium-mediated disc method. 65 Kan~R transgenic tobacco plants were regenerated. Then 5 transgenic tobacco plants expressing different levels of agglutinin were selected from these Kan~R plants through genomic PCR,ELISA,RT-PCR and Western-blot. A preliminary aphid bioassay experiment was carried out for investigation of the transgenic tobacco resistance to Myzus persicae,which showed that the aphid inhibition rate was significant,with the maximum reaching to 85.8%. Above results provided a theoretical basis for the research on breeding of crops resistant to aphid and the application of bivalent aphid resistance genes.更多还原