【摘要】 应用模式生物秀丽隐杆线虫（Caenorhabdites elegans）研究了在0.005～3.2 mg·L^-1范围内顺式氯氰菊酯（α-CPM）和高效氯氰菊酯（β-CPM）的暴露对机体生殖发育的影响,探讨了两种氯氰菊酯导致线虫生殖发育损伤的信号转导通路。结果表明,0.05 mg·L^-1的α-CPM和β-CPM暴露均诱导线虫发生显著的生殖细胞凋亡,具有剂量效应;生殖损伤信号的转导主要依赖JNK MAPK信号通路,部分依赖p38 MAPK信号途径。较低浓度的α-CPM和β-CPM暴露亦诱导线虫生殖腺的DNA损伤,主要表现在线虫产卵量的减少和卵孵化率的降低,并具有剂量效应。研究表明,α-CPM和β-CPM暴露均诱导显著的生殖细胞凋亡,且β-CPM生殖毒性较α-CPM大。更多还原

【Abstract】 In this study, Caenorhabdites elegans（C. elegans）was selected as a model organism with which to explore the effects of alphacypermethrin（α-CPM）and beta-cypermethrin（β-CPM）on reproduction from the concentrations of 0.005 to 3.2 mg·L^-1. The signal transduction pathways were also further investigated. The results showed that germ cell death and germline apoptosis in C. elegans was affected by α-CPM and β-CPM at 0.05 mg·L^-1, with dose effect. Further, the transduction of the reproductive injury signal was mainly dependent on the JNK MAPK signal transduction pathway and partially dependent on the p38 MAPK signal transduction pathway. Lower concentrations of α-CPM and β-CPM also induced DNA damage in the gonad of C. elegans, which mainly manifested as reduced brood size and hatching rate, with dose effect. These results indicate that exposure to α-CPM and β-CPM causes significant germ cell apoptosis in C. elegans. Also, the reproductive toxicity of β-CPM is higher than that of α-CPM.更多还原