【摘要】 为了给脐橙微生物肥料的研制和生产提供优良菌株,从赣南脐橙根际土壤中分离获得19株菌能产吲哚乙酸(Indole-3-Acetic Acid, IAA)的菌株,其中IAA-6菌株产IAA能力最强.对IAA-6菌株进行菌落形态观察和16S rDNA序列分析,利用分光光度法测定产IAA的能力,并对最佳碳氮源、产素等发酵条件进行了筛选.结果表明,IAA-6菌株菌落形态光滑、透明、黄色,初步命名IAA-6菌株为Pantoea sp. IAA-6,其最适发酵条件为p H为7,装液量为75 mL/250 mL,培养时间为29 h,碳源为淀粉,氮源为蛋白胨,IAA产量可达到33.299 mg/L.研究发现IAA-6菌株具有较强的IAA产生能力,具有作为脐橙功能微生物肥料研发的潜力,可进一步对其进行开发研究.更多还原

【Abstract】 In order to provide excellent strains for development and production of microbial fertilizer utilization in Gannan navel orange, a total of 19 Indole-3-Acetic Acid(IAA) producing bacteria were successfully isolated from the rhizosphere soil of Gannan navel orange. Among them, the IAA-6 strain had the highest IAA prod uctivity. The colony morphology and 16 S r DNA sequence was analyzed, the IAA productive ability was determined by using spectrophotometric method, and the fermentation optimization for IAA-6 strain wasd further carried out. The results show that the morphology of IAA-6 strain was smooth, transparent and yellow, and the IAA-6 strain was identified and named as Pantoea sp. IAA-6. The optimal fermentation conditions for IAA-6 strain were as follows: pH 7.0, a 75 mL medium in 250 mL Erlenmeyer flask, 29 h of incubation time, starch as the carbon source, and peptone as the nitrogen source, in which the IAA yield could reach 33.299 mg/L. Due to the efficient IAA production capacity, it could be concluded that the IAA-6 strain has the potential to be used as a functional microbial fertilizer for navel orange, and is value for further development and research.更多还原