【摘要】 目的探讨组织蛋白酶B(Cathepsin B,Cat B)在肿瘤坏死因子相关凋亡配体(TRAIL)诱导胃腺癌SGC-7901细胞凋亡的作用。方法采用MTT法检测不同浓度TRAIL对胃腺癌SGC-7901细胞增殖情况的影响;用不同浓度Cat B抑制剂和TRAIL共同诱导SGC-7901细胞48h,用流式细胞仪检测细胞凋亡率;用不同浓度Cat B抑制剂和TRAIL共同诱导SGC-7901细胞48h,用免疫印迹法(Western blot)检测Cat B表达的变化。结果 TRAIL对SGC-7901细胞增殖有抑制作用,最佳作用浓度为100μg/L;分别用0μmol/L、0.2μmol/L、0.5μmol/L、1.0μmol/L、2.0μmol/L、4.0μmol/LCA-074(Me)作用时,细胞凋亡率分别为36%、34%、28%、20%、18%、15%(P<0.05),Cat B抑制剂浓度越高,细胞凋亡率越低;Western blot结果表明,Cat B表达分别为(1.25±0.08)、(1.14±0.09)、(0.93±0.05)、(0.61±0.07)、(0.38±0.06)和(0.31±0.44)(P<0.05),Cat B抑制剂浓度越高,Cat B表达越少。结论组织蛋白酶B在TRAIL诱导胃腺癌SGC-7901细胞凋亡中具有调控作用。更多还原

【Abstract】 Objective To investigate the effect of cathepsin B(Ca B) on apoptosis of gastric adenocarcinoma SGC-7901 cells induced by tumor necrosis factor-related apoptosis ligand(TRAIL).Methods The effects of different concentrations of TRAIL on the proliferation of gastric adenocarcinoma SGC-7901 cells were detected by MTT assay. SGC-7901 cells were induced with different concentrations of Cat B inhibitor and TRAIL for 48 h, and the apoptosis rate was detected by flow cytometry. SGC-7901 cells were induced by Cat B inhibitor and TRAIL for 48 h, and the expression of Cat B was detected by Western blot.Results TRAIL inhibited the proliferation of SGC-7901 cells at an optimal concentration of 100μg/L; 0μmol/L, 0.2μmol/L, 0.5μmol/L, 1.0μmol/L, 2.0μmol/L, 4.0μmol/LCA, respectively. When-074(Me), the apoptotic rate was 36%, 34%, 28%, 20%, 18%, 15%(P<0.05). The higher the concentration of Cat B inhibitor, the higher the apoptosis rate. Low; Western blot results showed that Cat B expression was(1.25±0.08),(1.14±0.09),(0.93±0.05),(0.61±0.07),(0.38±0.06) and(0.31±0.44)(P<0.05), the higher the concentration of Cat B inhibitor, the less Cat B expression.Conclusion Cathepsin B has a regulatory role in TRAIL-induced apoptosis of gastric adenocarcinoma SGC-7901 cells.更多还原