【摘要】 为测定抑郁症小鼠海马中5-羟色胺（5-HT）、色氨酸（TRP）和5-羟吲哚乙酸（5-HIAA）,比较了两种测定方法的应用,即带荧光检测器的高效液相色谱法（HPLC-FLD）和超高效液相色谱-四极杆/轨道阱质谱法（UHPLC-Q-Obitrap-MS）的比较。样品的前处理选用0.4 mol·L^-1高氯酸溶液以1∶5（g∶mL）比例加入于小鼠海马组织的匀浆液中沉淀其中的蛋白,经涡旋和离心后取上清液,分别按上述两种方法进行测定。在两种方法中均采用C₁₈柱分离。试验结果表明:Q-Obitrap-MS测定显示较强的基质干扰,而HPLC-FLD测得的5-HT、TRP和5-HIAA的色谱峰形对称,峰宽窄,基质无明显干扰。3种目标物的质量浓度在500.0μg·L^-1以内呈线性。不同浓度水平的加标回收率为86.2%,92.1%,91.0%。上述3项测定目标物的检出限（3S/N）依次为1.0,1.0,0.5μg·L^-1。更多还原

【Abstract】 Two methods（i.e.HPLC with FLD and UHPLC-Q-Obitrap-MS）for determination of 5-hydroxytryptamine（5-HT）,tryptophan（TRP）and 5-hydroxyindole acetic acid（5-HIAA）in hippocampus of mouse affected by depression were studied comparatively.In the pretreatment of sample,0.4 mol·L^-1 HClO₄ solution was added to the homogenate of mouse hippocampus by the ratio of 1 to 5（g∶mL）to precipitate the protein.After swirling and centrafuging,the supernatants obtained by each of the 2 pretreatment method were used separately for analysis by the 2 methods.Separation on C₁₈column was adopted in both methods.As shown by the experimental results,strong matrix interference was encountered in the method of Q-Obitrap-MS,while symmetrical chromatographic peaks with narrow peak with for the 3 objective analytes were obtained and no significant matrix effect was observed by the method of HPLC-FLD,the standard curves of which showed linearity in the concentration range within 500.0μg·L^-1.Test for recovery was made by addition of standards at 3concentration levels,giving results of recovery of 86.2%,92.1%,and 91.0%.Detection limits（3S/N）found for the 3 analytes were 1.0,1.0,0.5μg·L^-1,respectively.更多还原