【摘要】 研究基于多巴胺(DA)对N-CQDs选择性荧光猝灭效应,实现了对多巴胺快速准确检测.研究以豆奶同时为碳源和氮源,通过一步水热法合成了具有高稳定性、高荧光产率的水溶性氮掺杂碳量子点(N-CQDs).利用透射电镜(TEM)、X射线光电子能谱(XPS)和傅里叶红外光谱(FI-IR)对N-CQDs进行表征。N-CQDs的粒径在3 nm左右,表面被-COOH、-NH2、-OH等官能团功能化,具有良好的水溶性.在优化条件下,DA对N-CQDs的猝灭作用与DA浓度在0. 1～100μmol/L内呈良好的线性关系(R2=0. 992 8),检出限为0. 05μmol/L(S/N=3),该方法用于尿液样品中DA的检测,回收率为93. 9%～109. 6%,相对标准偏差为2. 9%～5. 4%.该方法可以实现对多巴胺的检测.更多还原

【Abstract】 To study the selective fluorescence quenching effect of dopamine( DA) on N-CQDs and to achieve rapid and accurate detection of dopamine,soluble carbon-doped carbon quantum dots( N-CQDs) with high stability and high fluorescence yield were synthesized by one-step hydrothermal method using soy milk as both carbon and nitrogen source. N-CQDs were characterized by transmission electron microscopy( TEM),X-ray photoelectron spectroscopy( XPS) and Fourier infrared spectroscopy( FIIR). The particle size of N-CQDs was about 3 nm. The surface was functionalized with functional groups such as-COOH,-NH2,-OH,and it had good water solubility. Under optimized conditions,the quenching effect of DA on N-CQDs was linearly correlated with DA concentration in the range of 0. 1-100 μmol/L( R2= 0. 992 8). The detection limit was0. 05 μmol/L( S/N = 3). This method was used for the detection of DA in urine samples with a recovery rate of93. 9% ～ 109. 6% and a relative standard deviation of 2. 9% ～ 5. 4%. This method can be used to detect dopamine.更多还原