【摘要】 【目的】探究截形叶螨中Wolbachia与Spiroplasma这两种共生菌之间的竞争关系,评估叶螨交配类型对其寿命的影响。【方法】对不同共生菌感染类型的雌螨在不同交配类型以及不同日龄的情况下其体内共生菌滴度进行定量PCR检测,对不同交配类型的雌螨寿命进行记录,利用SPSS19.00(IBM)分析交配类型、成螨日龄对共生菌滴度的影响,以及叶螨交配类型对寿命的影响。【结果】不同交配类型以及不同日龄的雌螨,其体内共生菌滴度存在显著差异;对于Wolbachia滴度,单感染Wolbachia雌螨与双感染在4日龄、10日龄有差异;对于Spiroplasma滴度,单感染Spiroplasma雌螨均显著高于双感染;双感染中Wolbachia滴度显著高于Spiroplasma;双感染雌螨与同一感染或不感染品系雄螨交配后的存活时间长于与单感染Spiroplasma雄螨交配,单感染Spiroplasma雌螨与同一感染或不感染品系雄螨交配后的存活时间长于与双感染雄螨交配。【结论】交配类型和成螨日龄影响共生菌滴度;Wolbachia的存在抑制Spiroplasma;交配类型会对雌螨寿命产生影响。更多还原

【Abstract】 [Objectives] To investigate the competitive relationship between Wolbachia and Spiroplasma in Tetranychus truncates and the effect of mating preferences on longevity in these mites. [Methods] qPCR was used to measure symbiont titers in adult mites of different ages and mating preferences and the effects of mate choice on the longevity of female mites recorded.[Results] Symbiont titers were significantly different in mites with different mating preferences and ages. Wolbachia titers were significantly different between singly and doubly Wolbachia-infected lines at 4 and 10 days of age. The Spiroplasma titers of singly Spiroplasma-infected lines were significantly higher than those of doubly-infected lines. Wolbachia titers were significantly higher than Spiroplasma titers in doubly-infected lines. Doubly-infected female mites that mated with doubly Spiroplasma-infected, or uninfected, male mites, lived longer than those that mated with singly infected males. Singly Spiroplasma-infected female mites that mated with singly infected male mites, or uninfected male mites, lived longer than those that mated with doubly infected male mites. [Conclusion] Mating preference and age affected symbiont titer and the presence of Wolbachia greatly reduced Spiroplasma titer. Mating preferences affected the longevity of female mites.更多还原