【摘要】 目的运用生物信息学方法分析尪痹胶囊免疫相关通路及作用靶点,为尪痹胶囊的作用及机制研究提供思路和依据。方法在台湾中医药资料库中检索尪痹胶囊各组分中药含有的化学成分,在Pubchem中检索与这些化学成分相互作用的靶蛋白,将靶蛋白上传至生物信息分析软件IPA,经数据分析,得到靶蛋白免疫相关的生物学通路及作用靶点,对这些信息进行生物学解析。结果检索到尪痹胶囊的靶蛋白共232个,构建靶点网络,并分析尪痹胶囊作用的免疫相关通路,其中与细胞免疫相关的前五个通路为巨噬细胞移动抑制因子调控的固有免疫通路,活化和未活化的T淋巴细胞IL-2表达调节通路,淋巴细胞CD27信号转导通路,OX40信号通路,T淋巴细胞4-1BB信号转导通路。结论尪痹胶囊可以通过作用于JUN、NF-κB、NF-κBIA、RelA分子对免疫相关通路起到调节作用。更多还原

【Abstract】 Objective To analyze the immunological pathways and action targets of WangBi JiaoNang(WBJN) by using bioinformatic method,and provide ideas and basis for the immunomodulatory mechanism study of WBJN.Methods TCM Database @ Taiwan are searched for the chemical ingredients of WBJN prescription.Target protein related with each ingredient are found in Pubchem.Then,these target proteins are uploaded to bioinformatics analysis software.Through data analysis,immune-related biological pathways and targets are obtained,and the information is biologically analyzed.Results A total of 232 target proteins are retrieved from the WBJN,a target network is constructed,and the immune-related pathways of the WBJN are analyzed.The top 5 pathways associated with cellular immune are MIF regulation of innate immunity,regulation of IL-2 expression in active and anergic T lymphocytes,CD27 signaling in lymphocytes,OX40 signaling pathway and 4-1 BB signaling in T lymphocytes.Conclusion WBJN could regulate immune-related pathways by acting on JUN,NF-κB,NF-κBIA and RelA.更多还原