【摘要】 目的探讨程序性死亡受体1(PD-1)、T细胞免疫球蛋白黏蛋白分子3(TIM-3)在乳腺癌患者T淋巴细胞表面的表达,分析T淋巴细胞中PD-1、TIM-3之间的相关性及其与患者临床病理特征的关系。方法采用便利抽样法选取2017年1月至2018年6月在该院诊治的35例乳腺癌患者作为主要研究对象,手术后收集患者的肿瘤组织标本和癌旁组织标本,将两种标本剪碎消化后,采用非连续密度梯度离心法提取T淋巴细胞,通过实时荧光定量PCR技术(Real-time PCR)测定T淋巴细胞PD-1、TIM-3的mRNA相对表达量,同时通过Western blotting技术测定其蛋白表达量。结果乳腺癌患者T淋巴细胞中PD-1、TIM-3的mRNA表达量和蛋白表达量均高于癌旁组织淋巴细胞中的表达量。患者的T淋巴细胞中PD-1、TIM-3的mRNA表达量会随着年龄、肿瘤大小、肿瘤分级、淋巴结转移的程度加深而增加,差异均有统计学意义(P<0.05)。相关性分析,乳腺癌患者T淋巴细胞中PD-1与TIM-3的mRNA表达水平呈正相关(r=0.878,P<0.001)。结论 PD-1、TIM-3在乳腺癌中的高水平表达,与乳腺癌患者的年龄、肿瘤大小、肿瘤级别、淋巴结是否转移等临床病理特征密切相关,两者联合阻断对抑制乳腺癌的发生、发展、转移起着重要作用,为乳腺癌的治疗提供了一个新思路。更多还原

【Abstract】 Objective To investigate the expression of programmed death receptor 1(PD-1)and T cell immunoglobulin mucin 3(TIM-3)on the surface of T lymphocytes in breast cancer patients,and to analyze the correlation between PD-1 and TIM-3 in T lymphocytes and its relationship with clinicopathological features of the patients.Methods A total of 35 patients with breast cancer treated in this hospital from January 2017 to June 2018 were selected as the main research subjects by using the convenient sampling method.The tumor tissue specimens and paracancerous tissue specimens were collected after surgery,and the tumor tissue specimens were cut into pieces and digested.The T lymphocytes were extracted by non-continuous density gradient centrifugation.The relative mRNA expression levels of PD-1 and TIM-3 in T lymphocytes were determined by real-time quantitative PCR(qRT-PCR),and their protein expression levels were determined by Western blot.Results The mRNA and protein expression levels of PD-1 and TIM-3 in T lymphocytes of breast cancer patients were higher than those in adjacent lymphocytes.The mRNA expression levels of PD-1 and TIM-3 in T lymphocytes of patients and clinicopathological features analysis showed that the mRNA expression levels of PD-1 and TIM-3 in T lymphocytes of the patients were increased with age,tumor size,tumor grade,and lymph node metastasis degree deepening,and the differences were statistically significant(P<0.05).The correlation analysis showed that there was a positive correlation between PD-1 and TIM-3 mRNA expression levels in T lymphocytes of breast cancer patients(r=0.878,P<0.001).Conclusion PD-1 and TIM-3 are highly expressed in breast cancer,which is closely related to the clinicopathological features such as age,tumor size,tumor grade,and lymph node metastasis of breast cancer patients.The both combined block has an important role for the occurrence,development and metastasis of breast cancer cells,which provides a new thinking for the treatment of breast cancer.更多还原