【摘要】 目的探讨microRNA-21（miR-21）siRNA对宫颈癌Hela细胞周期和细胞凋亡的影响。方法实时荧光定量PCR检测Hela细胞转染后siRNA干扰组及阴性对照组中miR-21的表达量。流式细胞仪分析Hela细胞周期及凋亡率的变化。结果转染siRNA干扰载体后,实时荧光定量PCR检测Hela细胞中miR-21明显低于空白对照组及阴性对照组,差异有统计学意义（P<0.05）,空白对照组与阴性对照组相比,差异无统计学意义（P>0.05）;siRNA表达载体可明显增加细胞凋亡率,并显现出G₀/G₁期阻滞作用。结论miR-21siRNA干扰载体可以高效地抑制宫颈癌Hela细胞miR-21基因的表达,增强细胞的凋亡,并使细胞周期阻滞于G₀/G₁期。更多还原

【Abstract】 Objective To detect the influence miRNA-21 siRNA on cell cycle and apoptosis of cervical cancer Hela cell.Methods Real time fluorescence quantitative PCR(Real-PCR)was used to detect the expression of miRNA-21 in Hela cells after siRNA transfection and negative control group.Apoptosis and cell cycle of Hela cells were analyzed by flow cytometry.Results The relative level of miRNA-21 in Hela cells after siRNA transfection was significantly lower than that in blank control group and negative control group by RealPCR(P<0.05),but there was no different between blank control group and negative control group(P>0.05).The apoptosis rate of the siRNA group was all higher than that in the blank control group and the negative control group,and the cells were blocked at the G0/G1 phase.Conclusion miRNA-21 siRNA interference carrier could effectively inhibit the expression of miRNA-21 gene in cervical cancer Hela cells,enhance cell apoptosis,and block cell cycle in G0/G1 phase.更多还原