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42份云南玉米自交系基于SSR荧光标记的遗传多样性分析
Analysis of Genetic Diversity of 42 Maize Inbred Lines from Yunnan by Fluorescent Labeled SSR Marker
【摘要】 通过分析云南常用42份玉米自交系品种的遗传多样性,以及云南近些年新育成自交系的遗传背景,为云南地区玉米育种提供参考依据。利用30对SSR多态性引物,对42个玉米自交系品种进行等位基因多样性和聚类分析,共检测到277个等位基因变异,平均每个位点检测到的等位基因数为9.2个,变化范围4~17个,片段大小介于86~434 bp之间,每个SSR位点的多肽信息量(PIC)在0.7008~0.9979之间,平均为0.9712。基于UPGMA进行遗传聚类分析,供试材料分类不明显,遗传相似系数均大于0.82,遗传相似度较高。目前由于大部分的玉米自交系品种都由骨干自交系而来,品种资源狭窄、遗传多样性不够丰富,导致新育成的玉米品种遗传背景相似,血缘关系相近,因此亟需在品种资源的有效利用、资源评价、材料共享和交流等方面开展相关工作,促进玉米种业发展。
【Abstract】 The genetic diversity of 42 common maize inbred lines in Yunnan and the genetic background of newly bred inbred lines in Yunnan in recent years were analyzed for providing reference for maize breeding in Yunnan. The allele diversity and clustering of 42 maize inbred lines were analyzed by using 30 pairs of SSR primers. A total of 277 alleles were detected, with 9.2 alleles per locus and a range of 4~17, segment sizes ranging from 86~434 bp, and polypeptide information(PIC) per SSR locus at 0.7008~0.9979, an average of 0.9712. Genetic clustering analysis based on UPGMA showed that the genetic similarity coefficient was higher than 0.82. The reason for this is that most maize inbred lines are derived from backbone inbred lines, with narrow variety resources and insufficient genetic diversity, resulting in the new maize varieties with similar genetic background and close kinship relations. Therefore, it is urgent to carry out relevant work in the effective utilization, resource evaluation, material sharing and exchange of the resources of varieties to promote the development of the maize seed industry.
- 【文献出处】 江西农业学报 ,Acta Agriculturae Jiangxi , 编辑部邮箱 ,2019年10期
- 【分类号】S513
- 【被引频次】10
- 【下载频次】117