【摘要】 尿苷二磷酸葡萄糖焦磷酸化酶(UGPase)是灵芝多糖合成途径的关键酶,用电子克隆方法得到灵芝UGPase基因,并利用生物信息学软件对UGPase基因编码蛋白的理化性质、亚细胞定位、亲/疏水性、信号肽、蛋白结构域、蛋白高级结构以及系统进化树的构建等方面进行了预测和分析。结果表明:UGPase基因全长1691 bp,包含一个1515 bp的完整开放阅读框,编码504个氨基酸;该蛋白不存在信号肽和跨膜结构域,它是定位于细胞质内的一种亲水性稳定蛋白酶;它的高级结构主要是由α螺旋结构和无规则卷曲结构所组成;同源比对分析显示,该酶基因编码的氨基酸序列和其他动植物的UGPase酶相比在序列组成、高级结构方面具有一定的相似性。更多还原

【Abstract】 UDPglucose pyrophosphorylase(UGPase) is the key enzyme of Polysaccharide synthesis in Ganoderma lucidum. A UDPglucose pyrophosphorylase(UGPase) gene from Ganoderma lucidum was cloned in silico based on the corresponding Ganoderma lucidum EST sequence in NCBI datebase. The physicochemical property, subcellular localization, hydrophilicity/hydrophobicity, signal peptide, domains, secondary and tertiary structure of protein plus the functional domain and the constructed of phylogenetic tree were analyzed by bioinformatics tools. The results showed that the full-length of UGPase gene from Ganoderma lucidum was 1691 bp and it contained a 1515 bp complete ORF which encoded 504 amino acids. The protein does not exist in the signal peptide and the transmembrane domain. It is positioned within the cytoplasm of a hydrophilic protease. The high-level structure of the protein is composed by alpha helix and random coil structure. Homology comparison and phylogenetic analysis showed that the amino acid encoded by UGPase gene in Ganoderma lucidu was highly homologous with those encoded by UGPase gene in other species.更多还原