【摘要】 目的:比较小鼠非同步胚胎移植技术。方法:将小鼠不同发育阶段的着床前胚胎非同步移植到不同假孕受体小鼠的输卵管或子宫内,观察非同步胚胎移植对小鼠胚胎移植成功率和后代出生重的影响。结果:CD-1小鼠受精卵、2-细胞胚胎、桑椹胚和囊胚都可移植到假孕0.5d受体小鼠输卵管继续发育,妊娠受体在移植后19d出生小鼠,胚胎移植出生率分别为56.3%、62.5%、59.4%和58.3%。桑椹胚和囊胚也可移植到假孕2.5 d受体子宫继续发育,妊娠受体在移植后17 d出生小鼠胚胎移植出生率分别为57.5%和62.5%。囊胚移植假孕0.5 d受体输卵管的出生小鼠的出生重显著大于移植假孕2.5 d受体子宫的出生小鼠。结论:不同发育阶段的胚胎若移植相同的假孕受体,则妊娠受体有相同的妊娠期;相同发育阶段的胚胎若移植不同的假孕受体,则妊娠受体有不同的妊娠期,假孕时间短的受体有较长的妊娠期和显著增加的小鼠出生重。更多还原

【Abstract】 Objective : To investigate the asynchronous embryo transfer in mice. Methods : Preimplantation embryos at different developmental stages were asynchronously transferred into the unilateral oviduct or uterine horn of pseudopregnant recipient mice with different post-coitum days, and the effect of asynchronous embryo transfer on success rate of embryo transfer and newborn birth weight was evaluated. Results : All of the zygotes, 2-cell embryos, morula, and blastocysts could be transferred into unilateral oviduct of pseudopregnant 0.5 day recipients and developed to live newborns 19 days after embryo transfer. The birth rates of the transferred zygotes, 2-cell embryos, morula, and blastocysts were 56.3%, 62.5%, 59.4%, and 58.3%, respectively. Morula and blastocysts could still be nonsurgically transferred into unilateral uterine horns of pseudopregnant 2.5 day recipients and developed into live newborns 17 days after embryo transfer. The birth rates of the transferred morula and blastocysts were 57.5% and 62.5%, respectively. The birth weight of newborns from the transferred blastocysts into oviducts of pseudopregnant 0.5 day recipients was significantly increased than that of newborns from the transferred blastocysts into uterine horns of pseudopregnant 2.5 day recipients.Conclusion : If embryos at different developmental stages are transferred into recipients with the same post-coitum days, the pregnant recipients would have the same gestation. If embryos at the same developmental stage are transferred into recipients with different post-coitum days, the pregnant recipients would have different gestation. The pregnant recipients with short post-coitum days have longer gestation and significantly increased birth weight of newborns.更多还原