【摘要】 2018年1—2月,饲养于集美大学疫苗中心的日本鳗鲡(Anguilla japonica)出现了一定数量的死亡,从病鱼脾脏中分离到一株优势菌株,结合Biolog系统微生物鉴定和分离菌16S rRNA基因的测序结果,显示该分离菌为嗜水气单胞菌(Aeromonas hydrophila)。采集自然感染嗜水气单胞菌前后的日本鳗鲡肝、脾、肾和粘液为cDNA模板,采用荧光定量PCR(real-time PCR)法检测组织中Toll样受体3(tlr3)、肿瘤坏死因子(tnf-α)、C型溶菌酶(lysC)和环氧合酶(cox2)等免疫相关基因的表达变化。结果表明:日本鳗鲡自然感染嗜水气单胞菌后,tlr3、tnf-α、cox2均未发现显著性的变化,但lysC的含量在日本鳗鲡脾脏和肾脏中均出现显著性上调,即,自然感染嗜水气单胞菌的日本鳗鲡的抗感染免疫功能与C型溶菌酶密切相关。更多还原

【Abstract】 From January to February in 2018,some Japanese eel farmed in the vaccine center of Jimei University(Fujian Province,China)at 25 ℃ was infected by bacterial pathogensand showed a certain number of deaths.A dominant bacteria strain was isolated from the spleen of the diseased eels.By Biolog identification and 16 S rRNA gene sequence analysis,the strain was identified as Aeromonas hydrophila.Then,RNA from kidney,spleen,liver and mucus of 5 diseased and 5 uninfected eels were extracted to detect the expressions of tnf-α,lysC,cox2 and tlr3 genes by quantitative real-time PCR.The results showed that the expression of lysC was significantly up-regulated between the infected and uninfected eels in spleen and kidney,but no significant difference was found in tnf-α,cox2 and tlr3 genes.The result indicated that the C-type lysozyme was closely related to the anti-infection immune function of Japanese eels naturally infected by A. hydrophila.更多还原