【摘要】 为揭示梅花WRKY基因的功能,采用RT-PCR技术从梅花品种‘雪梅’(Prunus mume ‘Xuemei’)中分离得到1个WRKY转录因子基因PmWRKY40。序列分析结果显示,该基因cDNA全长1 072bp,完整开放阅读框972bp,编码323个氨基酸,包含1个WRKY结构和1个C2H2型锌指结构。系统进化分析结果显示,梅花PmWRKY40蛋白与欧洲甜樱桃亲缘关系最近。实时荧光定量PCR分析表明:PmWRKY40基因受低温、氧化胁迫诱导,但诱导程度存在差异。此外,SA处理显著提高PmWRKY40的表达,ABA处理则抑制该基因表达。推测PmWRKY40可能在梅花响应低温胁迫过程中起重要作用。更多还原

【Abstract】 WRKY transcription factor plays a vital role in response to cold stress in plants.To reveal the function of WRKY gene in Prunus mume,the PmWRKY40 gene was cloned from P.mume‘Xuemei’by RT-PCR.The full-length cDNA sequence of PmWRKY40 was 1 072 bp,containing a complete open reading frame(ORF)of 972 bp encoding 323 amino acids.The deduced amino acid sequence alignment of PmWRKY40 contained a conserved WRKY domain and C2 H2 zinc-finger structure.The results of phylogenetic analyses showed that the PmWRKY40 was most closely related to WRKY protein from P.avium.The results of quantitative PCR showed that PmWRKY40 was induced by cold and H2 O2 stresses,but the induction levels of the gene expression were different between cold and H2 O2 stress.Meanwhile,PmWRKY40 was significantly triggered by SA treatment and its expression was inhibited by ABA.It is indicated that PmWRKY40 fromP.mume may play a key role in response to cold stress.更多还原