【摘要】 利用cDNA末端快速克隆(RACE)技术,获得海带(Saccharina japonica) CRY-DASH基因(SjCRYDASH)全长序列,结构分析发现,其ORF区长1779 bp,编码592个氨基酸。进行氨基酸同源序列比对,其与其他藻类和高等植物间存在两个重要辅基MTHF和FAD结合的保守域。通过不同光质照射诱导海带幼孢子体,发现蓝光、白光诱导1h后,均能使SjCRY-DASH转录水平上升,且SjCRY-DASH对蓝光的响应更强烈。本研究结果为研究大型褐藻-海带CRY-DASH受光诱导调控功能打下基础。更多还原

【Abstract】 In the present work, we characterized the full-length cDNA of CRY-DASH from Saccharina japonica(designated as SjCRY-DASH) using the rapid amplification of cDNA ends technique. The open reading frame length of SjCRY-DASH was 1779 bp, and the gene encoded a protein of 592 amino acids. Using multiple alignments of protein sequences, conserved residues interacting with two crucial cofactors, MTHF and FAD, were detected between SjCRY-DASH and several DASH-type proteins of other algae and higher plants. Juvenile sporophytes were induced by light of different wavelengths. Both blue and white light upregulated the transcriptional level of SjCRY-DASH within 1 h, although the gene showed stronger responses to blue light than to white light. Our results lay a foundation for future studies on light induction and regulation by CRY-DASH of S. japonica.更多还原